MACROPHAGE-MEDIATED KILLING OF OPSONIZED TREPONEMA-PALLIDUM
MACROPHAGE-MEDIATED KILLING OF OPSONIZED TREPONEMA-PALLIDUM
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DOI：
10.1093/infdis/165.1.69
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发表时间：
1992-01-01
影响因子：
6.4
通讯作者：
LUKEHART, SA
中科院分区：
文献类型：
--
作者：
BAKERZANDER, SA;LUKEHART, SA
在体外实验中，证实了蛋白胨诱导的正常兔腹腔巨噬细胞对梅毒螺旋体亚种梅毒螺旋体的杀伤能力。将梅毒螺旋体和10%加热免疫血清和正常血清按1：200比例与巨噬细胞孵育。孵育2-10h后，在正常兔的重复部位皮内注射这些混合物。最大杀伤率(未形成皮损)出现在免疫血清孵育的10小时：与正常血清孵育后90%(9/10)相比，仅有7%(1/14)的皮损形成(P<；.001)。在免疫血清存在下，最大吞噬作用(免疫荧光法检测)发生在8h，此时90%的巨噬细胞吞噬了梅毒螺旋体。然而，在这一点上，与密螺旋体和免疫血清孵育的巨噬细胞仍有70%的病变部位形成，这表明有效的杀伤可能需要吞噬后至少2小时。
The ability of proteose peptone-induced normal rabbit peritoneal macrophages to kill Treponema pallidum subspecies pallidum in vitro is demonstrated. Treponemes and 10% heated immune or normal sera were incubated with macrophages at a ratio of 1:200. After 2-10 h of incubation, these mixtures were injected intradermally at duplicate sites on normal rabbits. Maximal killing (failure to develop lesions) was seen at 10 h of incubation with immune serum: Only 7% (1/14) of lesions developed compared with 90% (9/10) after incubation in the presence of normal serum (P < .001). Maximal phagocytosis (detected by immunofluorescence) occurred by 8 h in the presence of immune serum, when 90% of macrophages had ingested treponemes. At this point, however, 70% of lesion sites from macrophages incubated with treponemes and immune serum still developed, suggesting that effective killing may require at least 2 h after phagocytosis.