Identification and validation of an immune cell infiltrating score predicting survival in patients with lung adenocarcinoma
Identification and validation of an immune cell infiltrating score predicting survival in patients with lung adenocarcinoma
复制标题
预测肺腺癌患者生存的免疫细胞浸润评分的鉴定和验证
DOI：
10.1186/s12967-019-1964-6
复制
发表时间：
2019-07-08
影响因子：
7.4
通讯作者：
Wang, Qun
中科院分区：
文献类型：
--
作者：
Yang, Xiaodong;Shi, Yu;Wang, Qun
背景
免疫浸润可能预测肺癌的生存情况并具有临床意义。然而，基于肿瘤整体转录组的免疫图谱所衍生的免疫特征在肺腺癌中尚未系统地建立。我们旨在利用一种评估免疫浸润的新算法构建一个免疫细胞浸润评分，以改进肺腺癌的预后模型。
方法
来自基因表达综合数据库（Gene Expression Omnibus）和癌症基因组图谱（The Cancer Genome Atlas）的肺腺癌公共数据集被用作训练队列和验证队列。使用CIBERSORT算法估计每个样本中不同免疫细胞亚型的比例。通过最小绝对收缩和选择算子回归模型进一步开发免疫浸润评分。然后进一步探究该模型的预后价值和临床相关性。
结果
在训练队列的免疫细胞基础上建立了一个免疫浸润评分模型。在肺腺癌患者中，高分与显著更差的生存情况相关（P
BackgroundImmune infiltration may predict survival and have clinical significance in lung cancer. However, immune signatures derived from immune profiling based on bulk tumor transcriptomes have not been systematically established in lung adenocarcinoma. We aimed to construct an immune cell infiltrating score, using a new algorithm for evaluating immune infiltration, to improve the prognostic model of lung adenocarcinoma.MethodsPublic datasets of lung adenocarcinoma from the Gene Expression Omnibus and The Cancer Genome Atlas were adopted as the training and validation cohorts. Fractions of different immune cell subtypes in each sample were estimated using the CIBERSORT algorithm. The immune infiltrating score was further developed by a least absolute shrinkage and selection operator regression model. The prognostic value and clinical relationship of the model was then further explored.ResultsAn immune infiltrating score model was established on the basis of the immune cells in the training cohort. A high score was associated with significantly worse survival in patients with lung adenocarcinoma (P < 0.001). The prognostic value of the score was confirmed in the validation cohort. The immune infiltrating score could improve the accuracy of predictions of survival when combined with the staging system. Furthermore, the score was potentially associated with patient smoking status and histologic subtype of lung adenocarcinoma. Its possible association with the efficacy of adjuvant chemotherapy was not statistically significant.ConclusionThe immune cell infiltrating score has prognostic significance in predicting overall survival in patients with lung adenocarcinoma.