Three antimicrobials alter gut microbial communities and causing different mortality of brown planthopper, Nilaparvata lugens Stal
Three antimicrobials alter gut microbial communities and causing different mortality of brown planthopper, Nilaparvata lugens Stal
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DOI：
10.1016/j.pestbp.2021.104806
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发表时间：
2021-04-08
影响因子：
4.7
通讯作者：
Yu, Xiaoping
中科院分区：
文献类型：
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作者：
Song, Yang;Shi, Jiateng;Yu, Xiaoping
褐飞虱肠道中的共生菌对其寄主褐飞虱的营养利用和生长具有重要作用。用抗菌剂抑制共生菌对水稻褐飞虱的侵染是可行的。然而，抗菌剂对肠道微生物群的影响还没有完全阐明。在本研究中，我们发现，当BPHS分别接触托霉素、戊唑醇和中生霉素时，死亡率分别达到35.5%、33.1%和19.4%。不同抗菌剂和水处理的BPHS成虫肠道微生物群落结构有显著差异。抗菌剂通过减少非优势真菌的丰度来降低真菌多样性，通过抑制肠道中的优势菌不动杆菌来增加细菌多样性。肠道分类群的多样性取决于抗菌剂选择性的不同。在微生物绝对丰度方面，抗菌剂处理组肠道微生物群落总丰度降低，沙雷氏菌绝对丰度显著增加。总之，本研究丰富了抗菌剂处理下褐飞虱肠道微生物区系的知识，为提高抗菌剂防治褐飞虱害虫的效果提供了指导。
The symbionts in the gut of brown planthopper play an important role in the nutrition utilization and growth of their host, Nilaparvata lugens St?l (Hemiptera: Delphacidae). Controlling the BPH infection on rice by inhibiting the symbionts using antimicrobials is feasible. However, the impact of antimicrobials on the microbiome in the gut has not been fully elucidated. In this study, we found the mortality reached 35.5%, 33.1% and 19.4%, when BPHs were exposed to toyocamycin, tebuconazole, and zhongshengmycin, respectively. Significant differences were found between the structures of gut microbial communities in adult BPHs treated with different antimicrobials and water. The antimicrobials reduced the fungal diversity by reducing the non-dominant fungi abundance, and increased bacterial diversity by inhibiting the dominant bacteria Acinetobacter in the gut. The diversification of taxonomic groups in gut depended on the different selective stress of antimicrobials. For the microbial absolute abundance, the total microbial gut community abundance decreased under antimicrobial exposure, but the absolute abundance of Serratia significantly increased in the antimicrobial treatment group. Overall, our study enriched the knowledge of microbiomes in the gut of BPH under the antimicrobial treatment and provided guidelines to enhance the pest management effect of BPH by using antimicrobials.