Expression analysis of PIN-formed auxin efflux transporter genes in maize
Expression analysis of PIN-formed auxin efflux transporter genes in maize
复制标题
玉米中 PIN 形成的生长素外排转运蛋白基因的表达分析
DOI：
10.1080/15592324.2019.1632689
复制
发表时间：
2019-06-19
影响因子：
2.9
通讯作者：
Zhang, Juren
中科院分区：
文献类型：
--
作者：
Li, Zhaoxia;Li, Peng;Zhang, Juren
生长素的局部梯度在生长素信号传导、生长素介导的发育和非生物胁迫反应中起着决定性的作用。由PIN形成的生长素外排转运蛋白对决定生长素的运输方向和维持局部生长素浓度梯度非常重要。在这项研究中，所有的候选PIN基因从现有的玉米基因组序列数据库进行了鉴定和分类的基础上氨基酸相似性。以山东登海种业有限公司从7922和478杂交后代中选育的玉米自交系DH4866为母本，分析了这些PINs的表达模式。组织特异性表达模式表明，它们可能在不同的发展阶段，特别是在根系中发挥不同的作用。对4个玉米PIN1基因的启动子基序分析以及它们在NAA、低磷和PEG处理下的表达水平表明，ZmPIN1a和ZmPIN1b可能比ZmPIN1c和ZmPIN1d在根系生长调节和非生物胁迫响应中发挥更大的作用。对ZmPIN1a和ZmPIN1b转基因株系（DH4866）的分析表明，它们对根的发育和生长具有不同的影响，ZmPIN1a增加侧根的数量并抑制其伸长以形成发达的根系，而ZmPIN1b通过促进侧根和种子根的生长来增加根生物量。这些结果表明，玉米PIN1基因在玉米发育过程中和对非生物胁迫的响应中起着协调作用。
ABSTRACT The local auxin gradient has a decisive role in auxin signaling, auxin-mediated development and abiotic stress response. PINFORMED (PIN)-formed auxin efflux transporters are very important for determining the direction of auxin transport and maintaining a local auxin concentration gradient. In this study, all candidate PIN genes from the current maize genome sequence database were identified and categorized based on amino acid similarity. The expression pattern of these PINs was analyzed in maize inbred line DH4866, which was selected from the progeny of 7922 and 478, and served as the female parent line of many hybrids in Shandong Denghai Seeds Co Ltd (China). Tissue-specific expression patterns indicated that they may have different roles in different stages of development, especially in the root system. Promoter motif analysis of four maize PIN1 genes and their expression levels in response to NAA, low phosphate levels and PEG treatment indicated that ZmPIN1a and ZmPIN1b may contribute more than ZmPIN1c and ZmPIN1d to root growth regulation and abiotic stress response. Analysis of the ZmPIN1a and ZmPIN1b transgenic lines (in DH4866) indicated that they have different effects on root development and growth, with ZmPIN1a increasing the number of lateral roots and inhibiting their elongation to form a developed root system, while ZmPIN1b increases root biomass by promoting the growth of both lateral and seminal roots. These results indicated that maize PIN1 genes function in coordination during maize development and in response to abiotic stress.