Interleukin-8 expression and modulation in human preovulatory follicles and ovarian cells
Interleukin-8 expression and modulation in human preovulatory follicles and ovarian cells
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DOI：
10.1210/en.137.9.3762
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发表时间：
1996-09-01
期刊：
影响因子：
4.8
通讯作者：
Olive, DL
中科院分区：
文献类型：
--
作者：
Arici, A;Oral, E;Olive, DL
在排卵前，主导卵泡的卵泡膜层中的中性粒细胞数量显著增加。在排卵前也检测到人类卵泡液中中性粒细胞趋化活性的升高。我们假设白细胞介素-8（IL-8），一种中性粒细胞趋化因子/激活因子和一种有效的血管生成剂，可能是排卵功能中白细胞趋化性的重要调节剂。在这方面，我们研究了IL-8在接受体外受精-胚胎移植治疗的患者的卵泡液样品中以及在卵巢基质细胞和颗粒黄体细胞培养物中的表达和调节。hCG后样本（n = 101）为262 +/- 45 pg/ml（P = 0.001）。在hCG后样本中，单个卵泡中IL-8的浓度与卵泡的大小相关（r = 0.61; P = 0.02）。我们还观察到血清IL-8水平（22 +/- 3 pg/ml）和卵泡液水平（303 +/- 143 pg/ml）之间的相关性，在11例同时检测两者的患者中，梯度为14倍（r = 0.71; P = 0.01）。IL-8信使RNA（mRNA）和蛋白质在卵巢基质细胞培养物中组成性表达，IL-1 α和肿瘤坏死因子-α以时间和浓度依赖性方式增加其水平。hCG和LH诱导IL-8 mRNA表达和蛋白产生水平升高。颗粒蛋白细胞也表达IL-8 mRNA和蛋白，IL-1 α和肿瘤坏死因子-α可增加其表达水平。重要的是，孕酮抑制基质细胞和颗粒黄体细胞类型中基础和IL-1 α刺激的IL-8表达。总之，我们发现IL-8水平在排卵期卵泡液中升高，颗粒黄体细胞和卵巢基质细胞都表达mRNA并产生蛋白质，类固醇和营养激素对这些细胞培养物中IL-8的调节表明IL-8可能在排卵生理学中起重要作用，如适时的卵泡破裂和黄体的新血管形成。
Just before the time of ovulation, the number of neutrophils increases markedly in the thecal layer of the leading follicle. A preovulatory rise in chemotactic activity for neutrophils in human follicular fluid has also been detected. We hypothesized that interleukin-8 (IL-8), a neutrophil chemoattractant/activating factor and a potent angiogenic agent, may be an important modulator of leukocyte chemotaxis in ovulatory function. In this regard we investigated the expression and modulation of IL-8 in human follicular fluid samples from patients undergoing in vitro fertilization-embryo transfer therapy and in ovarian stromal and granulosa-lutein cell cultures.The concentration of IL-8 in pre-hCG follicular fluid samples (n = 4) was 16 +/- 12 (mean +/- SEM) pg/ml, and that in post-hCG samples (n = 101) was 262 +/- 45 pg/ml (P = 0.001). In post-hCG samples, the concentration of IL-8 in an individual follicle correlated with the size of that follicle (r = 0.61; P = 0.02). We also observed a correlation between serum IL-8 levels (22 +/- 3 pg/ml) and follicular fluid levels (303 +/- 143 pg/ml), with a 14-fold gradient (r = 0.71; P = 0.01) in 11 patients tested for both. IL-8 messenger RNA (mRNA) and the protein were expressed constitutively in ovarian stromal cell cultures, and the level was increased by IL-1 alpha and tumor necrosis factor-alpha in a time- and concentration-dependent manner. hCG and LH induced higher levels of IL-8 mRNA expression and protein production. Granulosalutein cells also expressed IL-8 mRNA and protein, and the levels were increased by IL-1 alpha and tumor necrosis factor-alpha. Importantly, progesterone suppressed both basal and IL-1 alpha-stimulated IL-8 expression in stromal and granulosa-lutein cell types.In summary, we found that IL-8 levels are elevated in periovulatory follicular fluid, and both granulosa-lutein and ovarian stromal cells express the mRNA and produce the protein, The modulation of IL-8 in these cell cultures by steroid and trophic hormones suggests that IL-8 may play an important role in the physiology of ovulation, such as timely follicular rupture and neovascularization of the corpus luteum.