Increased Dentate Gyrus Excitability in Neuroligin-2-Deficient Mice in Vivo
Increased Dentate Gyrus Excitability in Neuroligin-2-Deficient Mice in Vivo
复制标题
Neuroligin-2 缺陷小鼠体内齿状回兴奋性增加
DOI：
10.1093/cercor/bhq100
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发表时间：
2011-02-01
期刊：
影响因子：
3.7
通讯作者：
Schwarzacher, Stephan W.
中科院分区：
文献类型：
--
作者：
Jedlicka, Peter;Hoon, Mrinalini;Schwarzacher, Stephan W.
The postsynaptic adhesion protein neuroligin-2 (NL2) is selectively localized at inhibitory synapses. Here, we studied network activity in the dentate gyrus of NL2-deficient mice following perforant path (PP) stimulation in vivo. We found a strong increase in granule cell (GC) excitability. Furthermore, paired-pulse inhibition (PPI) of the population spike, a measure for gamma-aminobutyric acid (GABA)ergic network inhibition, was severely impaired and associated with reduced GABA(A) receptor (GABA(A)R)-mediated miniature inhibitory postsynaptic currents recorded from NL2-deficient GCs. In agreement with these functional data, the number of gephyrin and GABA(A)R clusters was significantly reduced in the absence of NL2, indicating a loss of synaptic GABA(A)Rs from the somata of GCs. Computer simulations of the dentate network showed that impairment of perisomatic inhibition is able to explain the electrophysiological changes observed in the dentate circuitry of NL2 knockout animals. Collectively, our data demonstrate for the first time that deletion of NL2 increases excitability of cortical neurons in the hippocampus of intact animals, most likely through impaired GABA(A)R clustering.
