Effect of variations in the conditions of DNA synthesis upon the accuracy of DNA replication.
Effect of variations in the conditions of DNA synthesis upon the accuracy of DNA replication.
复制标题
DNA 合成条件的变化对 DNA 复制准确性的影响。
DOI：
10.1007/978-1-4613-2449-2_11
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Sinha,NK
中科院分区：
文献类型：
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作者：
Razzaki,T;Sinha,NK
最近在测量DNA复制准确性的方法上的改进导致了对细菌和哺乳动物DNA聚合酶和forE在自然DNA复制过程中许多错对的准确估计。大肠杆菌和噬菌体T4多酶复制复合体（见参考文献17和25最近的评论）。使用噬菌体T4的七个纯化复制蛋白（基因32、41、43、44、45、61和62的产物）的组合在体外复制DNAin，研究表明，该复合物的准确性与ΦX174 DNAin在体内复制时非常相似[12,16,23 - 25]。在体内以低准确度复制的DNA序列（如asam33）在体外复制时显示出高错误率。其他在体内还原率较低的序列（如am16和am9）在体外的复制精度相对较高[23-25]。研究还表明，通过适当使用脱氧核苷酸池偏差，可以通过特定的过渡和翻转途径诱导给定琥珀突变体的逆转[12,23,24]。我们研究了在体外DNA复制条件下的各种扰动对导致过渡和翻转突变的错对形成率的影响。这些结果总结在这里。
Recent refinements in the methods for measurement of accuracy of DNA replication have led to accurate estimates for a number of mispairs during the duplication of natural DNA for bacterial and mammalian DNA polymerases and forE. coliand bacteriophage T4 multienzyme replication complexes (see Refs. 17 and 25 for recent reviews). Using a combination of seven purified replication proteins of phage T4 (the products of genes 32, 41, 43, 44, 45, 61, and 62) to replicate DNAin vitro, it has been shown that the accuracy of this complex is very similar to that seen during replication of ΦX174 DNAin vivo[12, 16, 23–25]. DNA sequences (such asam33) that are replicated with a low accuracyin vivoshow high error rates during replicationin vitro. Other sequences (such asam16 andam9), which have a low reversion ratein vivo, are replicated with comparably high accuracyin vitro[23–25]. It has also been shown that by the appropriate use of deoxynucleotide pool biases, it is possible to induce reversions of a given amber mutant by specific transition and transversion pathways [12, 23, 24]. We have examined the influence of a variety of perturbations in the conditions of DNA replicationin vitro, on the rates of formation of mispairs leading to transition and transversion mutations. These results are summarized here.