Accurate transcription initiation by RNA polymerase II from Candida utilis.
Accurate transcription initiation by RNA polymerase II from Candida utilis.
复制标题
来自产朊假丝酵母的 RNA 聚合酶 II 的准确转录起始。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
Rao Gr
中科院分区：
文献类型：
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作者：
Meera Patturajan;Dipankar Chatterji;Rao Gr
描述了产朊假丝酵母的体外转录系统。使用的模板是含有与合成的 377 bp G-minus casette 连接的酿酒酵母 CYC1 启动子的混合质粒 (1)。体外转录是在 RNase 存在的情况下进行的。 T1。在这些条件下，只有对 RNase T1 具有抗性的转录本才会积累。使用该方案，已表明在缺乏胞质因子的情况下，来自产朊球菌的 RNA 聚合酶 II (pol II) 随机启动 RNA 合成。但产朊球菌和酿酒酵母的无细胞提取物都可以指导来自产朊球菌的 pol II 准确启动转录。结果还表明，酿酒酵母和产朊酵母之间的通用转录因子在功能上是可以互换的。
An in vitro transcription system from Candida utilis is described. The template used is a hybrid plasmid containing Saccharomyces cerevisiae CYC1 promoter linked to a synthetic 377-bp G-minus casette (1). In vitro transcriptions are carried out in the presence of RNase. T1. Under these conditions only the transcripts that are resistant to RNase T1 accumulate. Using this protocol, it has been shown that in the absence of cytosolic factors RNA polymerase II (pol II) from C. utilis initiated RNA synthesis randomly. But both C. utilis and S. cerevisiae cell-free extracts could direct pol II from C. utilis to initiate transcription accurately. Results also indicated that the general transcription factors are functionally interchangeable between S. cerevisiae and C. utilis.