Expression of neolactoglycolipids: sialosyl-, disialosyl-, O-acetyldisialosyl- and fucosyl- derivatives of neolactotetraosyl ceramide and neolactohexaosyl ceramide in the developing cerebral cortex and cerebellum.
Expression of neolactoglycolipids: sialosyl-, disialosyl-, O-acetyldisialosyl- and fucosyl- derivatives of neolactotetraosyl ceramide and neolactohexaosyl ceramide in the developing cerebral cortex and cerebellum.
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新乳糖脂的表达：发育中的大脑皮层和小脑中新乳四糖基神经酰胺和新乳六糖基神经酰胺的唾液酸基-、二唾液酸基-、O-乙酰基二唾液酸基-和岩藻糖基-衍生物。
DOI：
10.1007/bf00731504
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发表时间：
1996
影响因子：
3
通讯作者：
Jungalwala,FB
中科院分区：
文献类型：
--
作者：
Chou,DK;Suzuki,Y;Jungalwala,FB
鉴定了以下新乳糖脂，并在大鼠大脑皮层和小脑中研究了它们的发育表达：Fucα1-3IIInLcOse4Cer、Fucα1-3VnLcOse6Cer 和 (Fuc)2α1-3III,3VnLcOse6Cer，以及酸性糖脂 NeuAcα2-3IVnLcOse4Cer [nLM1]、 (NeuAc)2α2-3IVnLcOse4Cer [nLD1]、O-乙酰基 (NeuAc)2α2-3IVnLcOse4Cer [OAc-nLD1] 及其高级新乳糖胺基同系物 NeuAcα2-3VInLcOse6Cer [nHM1] 和 (NeuAc)2α2-3VInLcOse6Cer [nHD1]。这些糖脂仅在胚胎阶段在大脑皮层中表达，并在出生后消失。这种损失归因于关键前体 LcOse3Cer 合成的下调，该前体是由乳糖神经酰胺：N-乙酰氨基葡萄糖转移酶合成的。另一方面，在小脑中，由于 LcOse3Cer 的可用性增加，这些糖脂随着出生后发育而增加。在小脑中，只有 nLM1 和岩藻糖基新乳糖脂在出生后 10-15 天后下降，这可能是由于其他糖基转移酶的调节。此外，在小脑中，nLD1 和 nHD1 被证明与分子层中的浦肯野细胞及其树突以及小脑深核中的轴突末端特异性相关，与之前显示的其他新乳糖脂类似。
The following neolacto glycolipids were identified and their developmental expression was studied in the rat cerebral cortex and cerebellum: Fucα1-3IIInLcOse4Cer,Fucα1-3VnLcOse6Cer and (Fuc)2α1-3III,3VnLcOse6Cer, as well as acidic glycolipids, NeuAcα2-3IVnLcOse4Cer [nLM1], (NeuAc)2α2-3IVnLcOse4Cer [nLD1],O-acetyl (NeuAc)2α2-3IVnLcOse4Cer [OAc-nLD1] and their higher neolactosaminyl homologues NeuAcα2-3VInLcOse6Cer [nHM1] and (NeuAc)2α2-3VInLcOse6Cer [nHD1]. These glycolipids were expressed in the cerebral cortex only during embryonic stages and disappeared postnatally. This loss was ascribed to the down regulation of the synthesis of the key precursor LcOse3Cer which is synthesized by the enzyme lactosylceramide:N-acetylglucosaminyl transferase. On the other hand in the cerebellum, these glycolipids increased with postnatal development due to increasing availability of LcOse3Cer. In the cerebellum, only nLM1 and fucosyl-neolactoglycolipids declined after postnatal day 10–15, perhaps due to regulation by other glycosyltransferases. Also, in the cerebellum, nLD1 and nHD1 were shown to be specifically associated with Purkinje cells and their dendrites in the molecular layer and with their axon terminals in the deep cerebellar nuclei, similar to other neolactoglycolipids shown previously.