The Ginkgo biloba extract EGb761 reduces stress sensitivity, ROS accumulation and expression of catalase and glutathione S-transferase 4 in Caenorhabditis elegans
The Ginkgo biloba extract EGb761 reduces stress sensitivity, ROS accumulation and expression of catalase and glutathione S-transferase 4 in Caenorhabditis elegans
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DOI：
10.1016/j.phrs.2006.11.006
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发表时间：
2007-02-01
影响因子：
9.3
通讯作者：
Kahl, R.
中科院分区：
文献类型：
--
作者：
Kampkoetter, A.;Pielarski, T.;Kahl, R.
银杏叶标准化提取物EGb 761是一种受欢迎的草药膳食补充剂，它被用作治疗各种脑血管疾病的植物药。EGb 761的有益影响被认为是由生理条件下以及对应激的反应中的多种生物学作用所赋予的。在这项研究中，我们研究了EGb 761在模式生物秀丽隐杆线虫中的作用。EGb 761能显著降低C.优美的在微量滴定板中进行的基于荧光的测定中，我们证明了EGb 761的保护作用，其通过增加对热应激的抵抗力和在单个活蠕虫中热应激条件下ROS积累的衰减来实现。在正常条件下，EGb 761延长了蠕虫的寿命，支持在应激条件下发现的有益效果。在报告基因的方法，使用个别活蠕虫的应激诱导型谷胱甘肽S-转移酶4的表达被证明是减少EGb 761在生理条件下，以及在氧化应激。EGb 761还导致胁迫诱导的过氧化氢酶基因的转录减少。这些结果表明，EGb 791对C.秀丽隐杆线虫的抗氧化能力至少部分归因于其缓解氧化应激的能力。(c)2006爱思唯尔有限公司保留所有权利。
The standardised extract EGb761 from the leaves of Ginkgo biloba is a popular herbal dietary supplement and it is used as a phytopharmacon for the therapy of diverse cerebral insufficiencies. The beneficial impact of EGb761 is believed to be conferred by diverse biological actions under physiological conditions as well as in response to stress. In this study we examined effects of EGb761 in the model organism Caenorhabditis elegans. EGb761 reduced the body size but did not affect the reproduction of C. elegans. In fluorescence-based assays performed in microtiter plates we demonstrated the protective action of EGb761 by the increase of resistance to thermal stress and the attenuation of ROS accumulation under conditions of thermal stress in single living worms. Under normal conditions the lifespan of the worms was extended by the EGb761 supporting the beneficial effects found under stress conditions. In a reporter gene approach using individual living worms the expression of the stress-inducible glutathione S-transferase 4 was shown to be reduced by EGb761 under physiological conditions as well as under oxidative stress. EGb761 also led to a decrease in transcription of the stress-inducible catalase genes. These results suggest that the beneficial impact of EGb791 on resistance to thermal stress and lifespan in C. elegans is at least partially due to its ability to relieve oxidative stress. (c) 2006 Elsevier Ltd. All rights reserved.