Light-based imaging of green fluorescent protein-positive ovarian cancer xenografts during therapy
Light-based imaging of green fluorescent protein-positive ovarian cancer xenografts during therapy
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DOI：
10.1006/gyno.2001.6297
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发表时间：
2001-09-01
影响因子：
4.7
通讯作者：
Zinn, KR
中科院分区：
文献类型：
--
作者：
Chaudhuri, TR;Mountz, JM;Zinn, KR
目标。本研究的目的是开发一种灵敏的、无创的成像方法，用于在治疗干预期间监测卵巢异种移植物。用编码绿色荧光蛋白（GFP）的复制缺陷腺病毒（Ad）载体感染人卵巢肿瘤细胞（SKOV3.ip1）。用荧光立体显微镜对gfp阳性肿瘤细胞进行体内和体外显像。使用合适的滤光片，同时检测GFP荧光和阿霉素。裸鼠植入gfp阳性细胞后，以无创方式反复成像。SKOV3。感染Ad-GFP的ip1细胞表现出高的GFP荧光，阿霉素处理后这种荧光被消除。GFP荧光缺失证实为死细胞。对于体内成像，在外部检测到直径小至0.2 mm的腹膜内肿瘤。通过体内显像检测肿瘤对阿霉素的摄取，肿瘤大小的减小与GFP荧光的降低同时发生。这些发现在尸检中得到证实。荧光立体显微镜监测卵巢异种移植物对阿霉素治疗的反应。首次同时对GFP和阿霉素进行成像。(C) 2001学术出版社。
Objective. The purpose of the study was to develop a sensitive, noninvasive imaging method for monitoring ovarian xenografts during therapeutic intervention.Methods. Human ovarian tumor cells (SKOV3.ip1) were infected with a replication-deficient adenoviral (Ad) vector encoding green fluorescent protein (GFP). The GFP-positive tumor cells were imaged in vitro and in vivo with a fluorescence stereomicroscope. Using appropriate filters, both GFP fluorescence and adriamycin were simultaneously detected. Nude mice implanted with GFP-positive cells were imaged repeatedly, in a noninvasive manner.Results. SKOV3.ip1 cells infected with Ad-GFP showed high GFP fluorescence, which was eliminated after treatment with adriamycin. Loss of GFP fluorescence was confirmed to be dead cells. For in vivo imaging, intraperitoneal tumors as small as 0.2 mm in diameter were detected externally. Adriamycin uptake was detected in tumors by in vivo imaging, and reduction in tumor size was concurrent with decrease in GFP fluorescence. These findings were confirmed at necropsy.Conclusions. Fluorescence stereomicroscopy monitored the response of ovarian xenografts to adriamycin therapy. For the first time, GFP and adriamycin were imaged simultaneously. (C) 2001 Academic Press.