SPAD Values and Nitrogen Nutrition Index for the Evaluation of Rice Nitrogen Status
SPAD Values and Nitrogen Nutrition Index for the Evaluation of Rice Nitrogen Status
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SPAD值和氮营养指数评价水稻氮素状况
DOI：
10.1626/pps.17.81
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
He, Junjun
中科院分区：
文献类型：
--
作者：
Yang, Hu;Yang, Jingping;He, Junjun
摘要 植物诊断是测定作物氮（N）含量的最重要方法之一。我们的目标是建立水稻三个发育阶段土壤-植物分析发展（SPAD）值和氮营养指数（NNI）之间的关系，并将SPAD仪用作预测谷物产量对施氮肥反应的诊断工具。我们测定了两个水稻品种在三个生长阶段、六种氮肥水平下四个最上面完全展开的叶子的SPAD值，并检验了SPAD值与NNI之间的关系。秀水 63 的临界氮浓度 (Nc) 为 5.31 W–0.5，杭 43 的临界氮浓度 (Nc) 为 5.38 W–0.49，其中 W 为地上部生物量。 SPAD值与NNI的相关性随叶位、发育阶段和品种的不同而变化。在生物量响应中，下部叶似乎比上部叶对氮水平更敏感，更适合作为氮状态诊断的测试样本，特别是在孕穗和抽穗期。在孕穗期，籽粒产量对第四片完全展开叶 (L4) SPAD 值的依赖性非常显着（2011 年 R2L4 = 0.82**，2012 年 R2L4 = 0.72**）。孕穗期L4与氮饱和图（RSPAD）（2011年R2L4 = 0.92**，2012年R2L4 = 0.77**）和NNI（2011年R2 = 0.96**，2012年R2 = 0.86**）SPAD值的比值表明与籽粒产量有更密切的关系。
Abstract Plant-based diagnosis is one of the most important methods to determine nitrogen (N) content of crops. Our objective was to establish the relationship between soil-plant analysis development (SPAD) values and N nutrition index (NNI) during the three developmental stages of rice and apply the SPAD meter as diagnostic tools for predicting grain yield response to N fertilization. We determined the SPAD values of four uppermost fully expanded leaves of two rice cultivars at six N fertilization levels at three growth stages and examined the relationship between SPAD values and NNI. The critical N concentration (Nc) was 5.31 W–0.5 in Xiushui63, and 5.38 W–0.49 in Hang43, where W is the total shoot biomass. The correlation between SPAD value and NNI varied with the leaf position, developmental stage, and variety. The lower leaf appeared to be more sensitive to the N level than the upper leaf in the response of biomass, and could be more suitable as a test sample for N status diagnosis, especially in the booting and heading stage. The dependence of grain yield on SPAD values of the fourth fully expanded leaf (L4) was significant at booting stage (R2L4 = 0.82** in 2011, R2L4 = 0.72** in 2012). Ratio of SPAD values of L4 to that in the N-saturated plot (RSPAD) (R2L4 = 0.92** in 2011, R2L4 = 0.77** in 2012) and NNI (R2 = 0.96** in 2011, R2 = 0.86** in 2012) at booting stage demonstrated a closer relationship with grain yield.