Maintaining a sense of direction during long-range communication on DNA.
Maintaining a sense of direction during long-range communication on DNA.
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DOI：
10.1042/bst0380404
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发表时间：
2010-04
影响因子：
3.9
通讯作者：
Seidel R
中科院分区：
文献类型：
--
作者：
Szczelkun MD;Friedhoff P;Seidel R
许多生物过程依赖于蛋白质与数千个碱基对分隔的多个DNA位点的相互作用。这些远程通信事件可以由蛋白质和DNA的热运动以及由ATP水解纠正的定向蛋白质运动驱动。本综述描述了相互矛盾的实验，这些实验试图解释atp依赖性III型限制性修饰酶如何能够切割具有两个反向重复位点的DNA，而不能切割具有两个直接重复位点的DNA。我们认为atp酶活性可能不会自动指示DNA转位酶，但可以指示通过热驱动DNA滑动触发通信的分子开关。本文还讨论了这种机制对其他依赖atp的通信过程（如错配修复）的通用性。
Many biological processes rely on the interaction of proteins with multiple DNA sites separated by thousands of base pairs. These long-range communication events can be driven by both the thermal motions of proteins and DNA, and directional protein motions that are rectified by ATP hydrolysis. The present review describes conflicting experiments that have sought to explain how the ATP-dependent Type III restriction–modification enzymes can cut DNA with two sites in an inverted repeat, but not DNA with two sites in direct repeat. We suggest that an ATPase activity may not automatically indicate a DNA translocase, but can alternatively indicate a molecular switch that triggers communication by thermally driven DNA sliding. The generality of this mechanism to other ATP-dependent communication processes such as mismatch repair is also discussed.