Expression of c-Fos, ICER, Krox-24 and JunB in the whisker-to-barrel pathway of rats: time course of induction upon whisker stimulation by tactile exploration of an enriched environment
Expression of c-Fos, ICER, Krox-24 and JunB in the whisker-to-barrel pathway of rats: time course of induction upon whisker stimulation by tactile exploration of an enriched environment
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DOI：
10.1016/s0891-0618(01)00155-7
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发表时间：
2002-03-01
影响因子：
2.8
通讯作者：
Staiger, JF
中科院分区：
文献类型：
--
作者：
Bisler, S;Schleicher, A;Staiger, JF
修正的触觉信息可诱导大鼠体感觉皮层的适应性可塑性。导致可塑性神经元反应的细胞机制。然而，它们在很大程度上是未知的。诱导型转录因子被认为是从修饰输入到改变神经元结构和功能的级联的一个主要环节。我们研究了转录因子诱导在大鼠须到桶通路的时空模式，通过将动物置于小说中。丰富的环境，同时在脸的一侧有修剪的胡须。这种刺激不仅在脑干小桶和丘脑类小桶中引起特异性的c-Fos诱导，而且在与桶相关的皮质柱中也引起特异性的c-Fos诱导，每种诱导的时间过程不同。在桶状皮质中，c-Fos和Krox-24免疫染色显示快速诱导，在1 h达到峰值，在14 h后恢复到基础水平。JunB在探索1 h后诱导。在6小时下降，并在此时间点后恢复到基础水平。诱导环AMP早期抑制因子（ICER）。cAMP信号通路的转录因子。在6点后达到最大值。下降缓慢，但5天后仍可检测到水平升高。我们的数据表明，在探索小说刺激须。丰富的环境。(i)在须-桶通路的皮层下中继站能够表达高水平的c-Fos， （ii）在桶皮层c-Fos， JunB， Krox-24和ICER在颞域受到差异调节。(C) 2002 Elsevier Science B.V.版权所有
Modified tactile information has been shown to induce adaptive plasticity in the somatosensory cortex of rat. The cellular mechanisms resulting in plastic neuronal responses. however, are largely unknown. Inducible transcription factors have been proposed as one major link in the cascade from modified input to altered neuronal structure and function. We investigated the spatial and temporal patterns of transcription factor induction in the rat whisker-to-barrel pathway by placing the animals in a novel. enriched environment while having clipped sets of whiskers on one side of the face. Such stimulation resulted not only in a specific c-Fos induction in brainstem barrelettes and thalamic barreloids, but also in the barrel-related cortical columns, each with different time courses. In the barrel cortex, c-Fos and Krox-24 immunostaining showed a rapid induction with peak levels at 1 h and a return to basal levels after 14 h. JunB was induced after 1 h of exploration. declined at 6 h and returned to basal levels after this time point. The inducible cyclic AMP early repressor (ICER). a transcription factor of the cAMP signaling pathway. showed a maximum after 6 It. decreased slowly, but elevated levels were still detectable after 5 days. Our data demonstrate that upon whisker stimulation by exploration of a novel. enriched environment. (i) subcortical relay stations in the whisker-to-barrel pathway are able to express elevated levels of c-Fos and (ii) in the barrel cortex c-Fos, JunB, Krox-24 and ICER are differentially regulated in the temporal domain. (C) 2002 Elsevier Science B.V. All rights reserved.