DIFFERENTIAL-EFFECTS OF EPIDERMAL GROWTH-FACTOR, SOMATOMEDIN-C INSULIN-LIKE GROWTH FACTOR-I, AND TRANSFORMING GROWTH FACTOR-BETA ON PORCINE GRANULOSA-CELL DEOXYRIBONUCLEIC-ACID SYNTHESIS AND CELL-PROLIFERATION
DIFFERENTIAL-EFFECTS OF EPIDERMAL GROWTH-FACTOR, SOMATOMEDIN-C INSULIN-LIKE GROWTH FACTOR-I, AND TRANSFORMING GROWTH FACTOR-BETA ON PORCINE GRANULOSA-CELL DEOXYRIBONUCLEIC-ACID SYNTHESIS AND CELL-PROLIFERATION
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DOI：
10.1210/endo-123-1-168
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发表时间：
1988-07-01
期刊：
影响因子：
4.8
通讯作者：
SCHOMBERG, DW
中科院分区：
文献类型：
--
作者：
MAY, JV;FROST, JP;SCHOMBERG, DW
最近的研究表明，哺乳动物卵巢可合成表皮生长因子（EGF）、生长激素c /胰岛素样生长因子I （Sm-C）和转化生长因子- β。（TGFb），这些生长因子可能在一定程度上构成卵巢内颗粒细胞增殖和分化调节的基础。本文所述的研究旨在确定EGF、Sm-C和TGFb在原代单层培养过程中在多大程度上调节DNA合成和颗粒细胞增殖。EGF，而不是Sm-C和TGFb，在一定条件下以剂量依赖的方式持续刺激猪颗粒细胞[3H]胸腺嘧啶的掺入（P < 0.01）。Sm-C （10 ng/ml）和TGFb （1 ng/ml）均能增强egf刺激的[3H]胸腺嘧啶掺入（分别为56%和300%）
Recent studies have suggested that the mammalian ovary synthesizes epidermal growth factor (EGF), somatomedin-C/insulin-like growth factor I (Sm-C), and transforming growth factor-.beta. (TGFb) and that these growth factors may in part form a basis for intraovarian regulation of granulosa cell proliferation and differentiation. The studies described herein were initiated to determine to what extent EGF, Sm-C, and TGFb function to regulate DNA synthesis and granulosa cell proliferation during primary monolayer culture. EGF, but neither Sm-C nor TGFb, alone consistently stimulated, in a dose-dependent manner, [3H]thymidine incorporation by porcine granulosa cells under defined conditions (P < 0.01). Sm-C (10 ng/ml) and TGFb (1 ng/ml) both enhanced EGF-stimulated [3H]thymidine incorporation (56% and 300%, respectively; P