Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays)
Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays)
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通过过度表达来自玉米（Zea mays）的应激反应性乙醛脱氢酶基因，显着提高烟草植物的应激耐受性
DOI：
10.1007/s11103-008-9382-9
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发表时间：
2008-11-01
影响因子：
5.1
通讯作者：
Liu, Yongsheng
中科院分区：
文献类型：
--
作者：
Huang, Weizao;Ma, Xinrong;Liu, Yongsheng
醛脱氢酶（ALDHs）在植物暴露于胁迫条件下产生的醛解毒过程中起着核心作用。为了鉴定禾本科作物玉米（Zea mays）胁迫应答所需的基因，分离并鉴定了一个ALDH （ZmALDH22A1）基因。ZmALDH22A1属于目前只在植物中发现的ALDH22家族。ZmALDH22A1编码一个593个氨基酸的蛋白，分别与Saccharum officinarum（95%）、Oryza sativa（89%）、Triticum aestivum（87%）和Arabidopsis thaliana（77%）的同源物具有高度同源性。Real-time PCR分析显示ZmALDH22A1在不同组织中的表达存在差异。幼苗根系在脱水、高盐度和ABA等非生物胁迫条件下，ZmALDH22A1表达水平均有不同程度的升高。camv35s启动子驱动ZmALDH22A1信号肽与黄色荧光蛋白（YFP）融合的番茄稳定构建体转化表明，该融合蛋白靶向质体。过表达ZmALDH22A1的转基因烟草表现出更高的抗逆性。转基因植物的抗逆性伴随着细胞脂质过氧化产生的丙二醛（MDA）的减少。
Aldehyde dehydrogenases (ALDHs) play a central role in detoxification processes of aldehydes generated in plants when exposed to the stressed conditions. In order to identify genes required for the stresses responses in the grass crop Zea mays, an ALDH (ZmALDH22A1) gene was isolated and characterized. ZmALDH22A1 belongs to the family ALDH22 that is currently known only in plants. The ZmALDH22A1 encodes a protein of 593 amino acids that shares high identity with the orthologs from Saccharum officinarum (95%), Oryza sativa (89%), Triticum aestivum (87%) and Arabidopsis thaliana (77%), respectively. Real-time PCR analysis indicates that ZmALDH22A1 is expressed differentially in different tissues. Various elevated levels of ZmALDH22A1 expression have been detected when the seedling roots exposed to abiotic stresses including dehydration, high salinity and abscisic acid (ABA). Tomato stable transformation of construct expressing the ZmALDH22A1 signal peptide fused with yellow fluorescent protein (YFP) driven by the CaMV35S-promoter reveals that the fusion protein is targeted to plastid. Transgenic tobacco plants overexpressing ZmALDH22A1 shows elevated stresses tolerance. Stresses tolerance in transgenic plants is accompanied by a reduction of malondialdehyde (MDA) derived from cellular lipid peroxidation.