Progesterone modulation of specific protein synthesis in the decidualized hamster uterus.
Progesterone modulation of specific protein synthesis in the decidualized hamster uterus.
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黄体酮对蜕膜仓鼠子宫中特定蛋白质合成的调节。
DOI：
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发表时间：
1983
影响因子：
3.6
通讯作者：
W. W. Leavitt
中科院分区：
文献类型：
--
作者：
Richard G. MacDonald;Kathryn O. Morency;W. W. Leavitt
在去个体化仓鼠子宫假妊娠的第6,7和8天，研究了孕酮依赖性特异性蛋白的合成。切除卵巢动物皮下类固醇植入物的去除诱导黄体酮戒断；保留植入物的仓鼠作为对照组。激素停用8小时后，体外培养1小时，用[35S]蛋氨酸标记子宫肌层和子宫蜕膜组织，用二维凝胶电泳分离蛋白质，然后对干燥的凝胶进行放射自显影。二维凝胶模式（图）分析显示，与对照组相比，使用黄体酮的样本放射自显像斑点强度增加和减少，表明黄体酮既抑制又刺激去个体化子宫的基因表达。相比于子宫肌层，蜕膜瘤的蛋白质合成对激素的戒断反应更强；例如，第7天的变化分别为45次和26次。每个组织中通过改变合成来响应黄体酮停药的蛋白质组被称为“结构域”。在去个体化过程中，黄体酮结构域的大小和重叠程度每天都在变化（不同日子该结构域共同的蛋白质合成变化）。尽管两种组织的细胞质中重叠的程度相似，但细胞核中反应的重叠却有明显的差异。在蜕膜核中有广泛的重叠，其中六个蛋白点在每天去除黄体酮后强度增加。相反，在肌内膜核的孕酮结构域之间很少有重叠。这些发现强调了这两种组织对黄体酮停药反应的差异，但两种组织中有五种蛋白质（一种细胞质蛋白，四种核蛋白）对激素去除的反应相似。这些研究表明：1)假妊娠期间孕酮在子宫中的支配改变了蜕膜瘤和子宫肌层中许多蛋白质的合成；2)黄体酮对脱个体化过程中特定蛋白质合成的影响有正负两个方向；3)黄体酮调节的蛋白质，以及受该激素影响的基因在蜕膜瘤和子宫肌层中主要是不同的；4)黄体酮对蜕膜子宫中特定蛋白质合成的调节是时间依赖性的，因此反应域的组成、大小和重叠程度每天都在变化。
Progesterone-dependent synthesis of specific proteins was studied on Days 6, 7 and 8 of pseudopregnancy in the decidualized hamster uterus. Progesterone withdrawal was induced by removal of subcutaneous steroid implants from ovariectomized animals; hamsters retaining the implants served as controls. Eight hours after hormone withdrawal, myometrial and deciduomal tissues were labeled with [35S]methionine during incubation for 1 h in vitro, and proteins were separated by two-dimensional gel electrophoresis followed by autoradiography of the dried gels. Analysis of the two-dimensional gel patterns (maps) revealed both increases and decreases in autoradiographic spot intensity comparing progesterone-withdrawn samples to controls, indicating that progesterone both suppressed and stimulated gene expression in the decidualized uterus. Protein synthesis in the deciduoma was more responsive to hormone withdrawal than that in the myometrium; e.g., 45 changes on Day 7 vs. 26 changes, respectively. The sets of proteins in each tissue responding to progesterone withdrawal through changes in synthesis have been termed "domains." The progesterone domains shifted from day to day during decidualization in size and degree of overlap (changes in protein synthesis common to the domain on different days). Although the degree of overlap was similar in cytosol from both tissues, marked differences were apparent in the overlap of responses in the nucleus. Overlap was extensive in the deciduomal nucleus where six protein spots increased intensity following progesterone removal on each day. In contrast, little overlap was seen between the progesterone domains in the myometrial nucleus. These findings emphasize the differences between these two tissues in their responses to progesterone withdrawal, yet five proteins (one cytosolic, four nuclear) responded similarly to hormone removal in both tissues. These studies show that: 1) progesterone domination of the uterus during pseudopregnancy alters synthesis of many proteins in both the deciduoma and myometrium; 2) progesterone effects on specific protein synthesis during decidualization occur in both positive and negative directions; 3) the proteins modulated by progesterone and, by extension, the genes affected by this hormone are predominantly different in the deciduoma and myometrium, and 4) progesterone modulation of specific protein synthesis in the decidualized uterus is time-dependent, such that the domains of response vary daily in composition, size and overlap.
类固醇激素对卵清蛋白和伴清蛋白基因转录的调节。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Palmiter,RD;Mulvihill,ER;Shepherd,JH;McKnight,GS
通讯作者：
McKnight,GS
通过二维凝胶电泳比较几种人类肿瘤和正常细胞的核蛋白。
影响因子：
11.2
作者：
Wu,BC;Spohn,WH;Busch,H
通讯作者：
Busch,H