Dynamics of seedling growth acclimation towards altered light conditions can be quantified via GROWSCREEN:: a setup and procedure designed for rapid optical phenotyping of different plant species
Dynamics of seedling growth acclimation towards altered light conditions can be quantified via GROWSCREEN:: a setup and procedure designed for rapid optical phenotyping of different plant species
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DOI：
10.1111/j.1469-8137.2007.02002.x
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发表时间：
2007-01-01
期刊：
影响因子：
9.4
通讯作者：
Schurr, Uli
中科院分区：
文献类型：
--
作者：
Walter, Achim;Scharr, Hanno;Schurr, Uli
使用一种新的设置，我们评估了烟草幼苗对光照条件变化的反应速度，并调查了拟南芥无淀粉突变体在早期发育阶段是否表现出生长潜力下降。叶面积和相对生长速度是基于自动放置在120个幼苗阵列上方的相机拍摄的照片来测量的。通过色调、饱和度和数值等参数阈值的彩色图像全局分割来检测幼苗的总叶面积。在暴露于高养分有效性的烟草中，相对生长速率在1d内对改变的光照条件发生动态驯化，而在暴露于低养分有效性的植物中不发生动态适应。叶面积的增加与烟地上部鲜重和干重的增加以及根的生长密切相关。相对生长速率比叶面积更能反映植物的动态生长习性。由于生长反应在早期发育阶段通常是最灵活的，这里描述的程序是朝着快速检测调节植物生长的内部(遗传)和外部(环境)参数变化的影响的标准化方案迈出的重要一步。
Using a novel setup, we assessed how fast growth of Nicotiana tabacum seedlings responds to alterations in the light regime and investigated whether starch-free mutants of Arabidopsis thaliana show decreased growth potential at an early developmental stage.Leaf area and relative growth rate were measured based on pictures from a camera automatically placed above an array of 120 seedlings. Detection of total seedling leaf area was performed via global segmentation of colour images for preset thresholds of the parameters hue, saturation and value.Dynamic acclimation of relative growth rate towards altered light conditions occurred within 1 d in N. tabacum exposed to high nutrient availability, but not in plants exposed to low nutrient availability. Increased leaf area was correlated with an increase in shoot fresh and dry weight as well as root growth in N. tabacum. Relative growth rate was shown to be a more appropriate parameter than leaf area for detection of dynamic growth acclimation. Clear differences in leaf growth activity were also observed for A. thaliana.As growth responses are generally most flexible in early developmental stages, the procedure described here is an important step towards standardized protocols for rapid detection of the effects of changes in internal (genetic) and external (environmental) parameters regulating plant growth.