Characterization of cDNAs induced in meiotic prophase in lily microsporocytes.
Characterization of cDNAs induced in meiotic prophase in lily microsporocytes.
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DOI：
10.1093/dnares/1.1.15
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发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
Tabata, S
中科院分区：
文献类型：
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作者：
Kobayashi, T;Kobayashi, E;Tabata, S
为了鉴定和分析高等植物生殖细胞形成过程中起作用的基因，分离并表征了含有减数分裂前期诱导信息的cDNA。用减数分裂前期特异的减数探针筛选长花百合减数分裂前期小孢子母细胞构建的 cDNA 文库。通过交叉杂交和部分测序将所选克隆分为18组。 Northern blot分析显示，对应于各个cDNA组的转录本在减数分裂的早期阶段开始积累，并在减数分裂和孢子形成过程中表现出克隆特异性特征。预测的基因产物的氨基酸序列显示出与已知基因产物的相似性，例如热休克蛋白、芽孢杆菌中的丝氨酸蛋白酶和酵母中的 RAD 51 基因产物。一半的推定基因产物具有疏水性 N 末端区域，表明它们可能充当信号肽。
To identify and analyze genes functioning during reproductive cell formation in higher plants, cDNAs harboring the messages induced in meiotic prophase were isolated and characterized. A cDNA library constructed from microsporocytes in meiotic prophase of Lilium longiflorum was screened with a subtraction probe specific to meiotic prophase. Clones selected were classified into 18 groups by cross hybridization and partial sequencing. Northern blot analysis revealed that the transcripts corresponding to the respective cDNA groups began accumulating at the early stages of meiosis and exhibited clone-specific profiles during meiosis and the spore formation process. The amino acid sequences of the predicted gene products showed similarity with known gene products, e.g. heat shock proteins, serine proteases in Bacillus, and RAD 51 gene product in yeast. Half of the putative gene products had hydrophobic N-terminal regions, suggesting that they may function as signal peptides.