Immune response in pigs vaccinated with plasmid DNA encoding ORF5 of porcine reproductive and respiratory syndrome virus
Immune response in pigs vaccinated with plasmid DNA encoding ORF5 of porcine reproductive and respiratory syndrome virus
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DOI：
10.1099/0022-1317-79-5-989
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发表时间：
1998-05-01
影响因子：
3.8
通讯作者：
Dea, S
中科院分区：
文献类型：
--
作者：
Pirzadeh, B;Dea, S
猪繁殖与呼吸综合征病毒(PRRSV)ORF5编码的主要囊膜糖蛋白(GP(5))是该病毒的三大结构蛋白之一。虽然一些猪恢复期血清和针对GP(4)和GP(5)的单抗在体外具有中和病毒的能力，但猪中和血清的蛋白质特异性尚未确定。在人巨细胞病毒启动子的控制下，PRRSV gp(5)基因表达载体DNA免疫猪和BALB/c小鼠产生抗gp(5)特异性中和抗体。免疫印迹和酶联免疫吸附试验证实中和血清具有GP(5)蛋白特异性。从DNA疫苗猪外周血中获得的单个核细胞在大肠杆菌表达的重组ORF5编码蛋白的存在下进行了胚胎性转化，表明了对GP(5)的细胞免疫反应的特异性。在对PRRSV毒株IAF-Klop进行大规模气管内攻击后，DNA疫苗接种的猪免受全身性病毒血症和典型肉眼观察到的肺部病变的发展，这在未接种疫苗的病毒挑战对照组以及用大肠杆菌表达的GST-ORF5重组融合蛋白免疫的猪中观察到。间质性肺炎和支气管肺泡炎在DNA疫苗接种的动物中明显较轻。这些结果表明，PRRSV的GP(5)是一种很好的亚单位重组疫苗候选株。
The ORF5-encoded major envelope glycoprotein (GP(5)) of porcine reproductive and respiratory syndrome virus (PRRSV) is one of the three major structural proteins of this virus. While some porcine convalescent sera and monoclonal antibodies directed against GP(4) and GP(5) have the capacity to neutralize the virus in vitro, the protein specificity of porcine neutralizing sera has not yet been established. DNA immunization with a plasmid encoding GP(5) of PRRSV, under the control of a human cytomegalovirus promoter, induced anti-GP(5)-specific neutralizing antibodies in pigs and BALB/c mice. The GP(5) protein specificity of neutralizing sera was confirmed by immunoblotting and ELISA. Peripheral blood mononuclear cells obtained from DNA-vaccinated pigs underwent blastogenic transformation in the presence of E. coli-expressed recombinant ORF5-encoded protein, indicating the specificity of the cellular immune response to GP(5). Following a massive intratracheal challenge with the virulent IAF-Klop strain of PRRSV, DNA-vaccinated pigs were protected from generalized viraemia and the development of typical macroscopic lung lesions that were observed in unvaccinated, virus-challenged controls, as well as in pigs that were immunized with E. coli-expressed GST-ORF5 recombinant fusion protein. Interstitial pneumonitis and broncho-alveolitis were remarkably milder in DNA-vaccinated animals. These results suggest that the GP(5) of PRRSV is a good candidate for a subunit recombinant-type vaccine.