Modulation of acetylcholine, capsaicin and substance P effects by histamine H3 receptors in isolated perfused rabbit lungs.
Modulation of acetylcholine, capsaicin and substance P effects by histamine H3 receptors in isolated perfused rabbit lungs.
复制标题
离体灌注兔肺中组胺 H3 受体对乙酰胆碱、辣椒素和 P 物质的调节作用。
DOI：
10.1016/0014-2999(95)00085-y
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发表时间：
1995
影响因子：
5
通讯作者：
M. Ansay
中科院分区：
文献类型：
--
作者：
A. Delaunois;P. Gustin;M. Garbarg;M. Ansay
在离体、通气的兔肺中研究了组胺 H3 受体在乙酰胆碱、辣椒素和外源性 P 物质诱导的肺水肿中的调节作用。通过测量毛细血管滤过系数（Kf，c）来评估内皮渗透性。乙酰胆碱（10−8至10−4M）、P物质（10−10至10−6M）、辣椒素（10−4M）和5-羟色胺（5-HT）（10−4M）诱导Kf,c增加。卡波酰胺是一种新型组胺 H3 受体拮抗剂，可引起乙酰胆碱的浓度-反应曲线显着左移，并增强辣椒素对 Kf,c 的作用，而对 P 物质和 5-HT 的反应没有显着影响。 Imetit 是一种新型组胺 H3 受体激动剂，可强烈抑制乙酰胆碱和辣椒素的作用。 Imetit 还强烈保护肺部免受 P 物质的影响，但不能阻止 5-HT 诱导的 Kf,c 增加。 Carboperamide 完全阻断 Imetit 对乙酰胆碱反应的抑制作用。 (R)-α-甲基组胺是另一种组胺 H3 受体激动剂，对乙酰胆碱反应具有与 Imetit 相同的保护作用。我们得出的结论是，组胺 H3 受体可以通过对 C 纤维的交界前调节作用，保护肺部免受乙酰胆碱和辣椒素诱导的水肿。然而，由于对外源性 P 物质的反应也受到组胺 H3 受体刺激的抑制，因此也表明突触后水平（可能在肥大细胞上）存在此类受体。
The modulatory role of histamine H3receptors in pulmonary oedema induced by acetylcholine, capsaicin and by exogenous substance P was investigated in isolated, ventilated rabbit lungs. Endothelial permeability was evaluated by measuring the capillary filtration coefficient (Kf,c). Acetylcholine (10−8to 10−4M), substance P (10−10to 10−6M), capsaicin (10−4M) and 5-hydroxytryptamine (5-HT) (10−4M) induced an increase in the Kf,c. Carboperamide, a novel histamine H3receptor antagonist, induced a significant leftward shift of the concentration-response curve to acetylcholine and also enhanced the effect of capsaicin on the Kf,c, while it had no significant effect on the response to substance P and 5-HT. Imetit, a new histamine H3receptor agonist, strongly inhibited the effects of acetylcholine and capsaicin. Imetit also strongly protected the lung against substance P effects but did not prevent the 5-HT-induced increase in the Kf,c. Carboperamide completely blocked the inhibitory effect of Imetit on the acetylcholine response. (R)-α-Methylhistamine, an other histamine H3receptor agonist, had the same protective effect against acetylcholine response as Imetit. We conclude that histamine H3receptors could protect the lung against acetylcholine- and capsaicin-induced oedema via a prejunctional modulatory effect on the C-fibres. However, since the response to exogenous substance P was also inhibited by histamine H3receptor stimulation, the presence of such receptors at a postsynaptic level, probably on mast cells, was also suggested.
豚鼠气管白三烯 D4 释放 P 物质。
影响因子：
1.7
作者：
Bloomquist,EI;Kream,RM
通讯作者：
Kream,RM