Jasmonic Acid-Induced VQ-Motif-Containing Protein OsVQ13 Influences the OsWRKY45 Signaling Pathway and Grain Size by Associating with OsMPK6 in Rice
Jasmonic Acid-Induced VQ-Motif-Containing Protein OsVQ13 Influences the OsWRKY45 Signaling Pathway and Grain Size by Associating with OsMPK6 in Rice
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DOI：
10.3390/ijms20122917
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发表时间：
2019-06-02
影响因子：
5.6
通讯作者：
Gomi, Kenji
中科院分区：
文献类型：
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作者：
Uji, Yuya;Kashihara, Keita;Gomi, Kenji
茉莉酸（JA）是一种植物激素，在水稻的防御反应和稳定生长中起着重要作用。在这项研究中，我们研究了JA响应缬氨酸谷氨酰胺（VQ）基序的蛋白OsVQ 13在水稻JA信号转导中的作用。OsVQ 13主要定位于细胞核和细胞质中。过量表达OsVQ 13的转基因水稻表现出对茉莉酸过敏的表型，并增强了茉莉酸诱导的对黄单胞菌的抗性。白叶枯病菌（Xoo）是引起水稻白叶枯病的细菌，是水稻上最严重的病害之一。此外，我们确定了丝裂原活化蛋白激酶，OsMPK 6，作为一个OsVQ 13相关蛋白。OsWRKY 45是一种重要的WRKY型转录因子，已知其受OsMPK 6调控，OsWRKY 45调控的基因在OsVQ 13过表达的水稻植株中表达上调。过量表达OsVQ 13的水稻植株的谷粒尺寸也大于野生型。这些结果表明，OsVQ 13通过激活水稻中的OsMPK 6-OsWRKY 45信号传导途径来正调控JA信号传导。
Jasmonic acid (JA) is a plant hormone that plays an important role in the defense response and stable growth of rice. In this study, we investigated the role of the JA-responsive valine-glutamine (VQ)-motif-containing protein OsVQ13 in JA signaling in rice. OsVQ13 was primarily located in the nucleus and cytoplasm. The transgenic rice plants overexpressing OsVQ13 exhibited a JA-hypersensitive phenotype and increased JA-induced resistance to Xanthomonas oryzae pv. oryzae (Xoo), which is the bacteria that causes rice bacterial blight, one of the most serious diseases in rice. Furthermore, we identified a mitogen-activated protein kinase, OsMPK6, as an OsVQ13-associating protein. The expression of genes regulated by OsWRKY45, an important WRKY-type transcription factor for Xoo resistance that is known to be regulated by OsMPK6, was upregulated in OsVQ13-overexpressing rice plants. The grain size of OsVQ13-overexpressing rice plants was also larger than that of the wild type. These results indicated that OsVQ13 positively regulated JA signaling by activating the OsMPK6-OsWRKY45 signaling pathway in rice.