Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans
Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans
复制标题
变形链球菌葡聚糖酶的结晶和初步晶体学分析
DOI：
10.1107/s1744309111038425
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发表时间：
2011
影响因子：
0.9
通讯作者：
Mori H,Kimura A
中科院分区：
文献类型：
--
作者：
Suzuki N;Kim YM;Fujimoto Z;Momma M;Kang HK;Funane K;Okuyama M;Mori H,Kimura A
变形链球菌葡聚糖酶水解葡聚糖的内部α-1，6-键，属于糖苷水解酶家族66。一种S.变形葡聚糖酶通过坐滴气相扩散法结晶。晶体的衍射分辨率为1.6 nm，空间群为P21，晶胞参数a = 53.2，B = 89.7，c = 63.3 nm，β = 102.3°。 假设晶体的不对称单元包含一个分子，则计算出的马修斯系数为4.07 3 Da−1;假设不对称单元中存在两个分子，则计算出的系数为2.03 3 Da−1。
Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P21, with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficient was calculated to be 4.07 Å3 Da−1; assuming the presence of two molecules in the asymmetric unit it was calculated to be 2.03 Å3 Da−1.