CELL TYPE-SPECIFIC GENE-EXPRESSION IN THE DROSOPHILA-MELANOGASTER MALE ACCESSORY-GLAND
CELL TYPE-SPECIFIC GENE-EXPRESSION IN THE DROSOPHILA-MELANOGASTER MALE ACCESSORY-GLAND
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DOI：
10.1016/0925-4773(92)90036-j
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发表时间：
1992-07-01
影响因子：
2.6
通讯作者：
WOLFNER, MF
中科院分区：
文献类型：
--
作者：
BERTRAM, MJ;AKERKAR, GA;WOLFNER, MF
雄性果蝇的附腺在生殖过程中起着重要的作用。这个分泌器官合成的产品被转移到女性，是必要的，以引起适当的生理和行为反应的女性。附腺由两种形态上不同的分泌细胞组成，即主细胞和次级细胞。先前的研究发现了一些基因在主细胞或所有的副腺细胞中表达。在本文中，我们使用P-元件介导的增强子陷阱，检查基因表达的附属腺。我们发现，除了在主细胞中表达的基因，只有或在所有的附属腺分泌细胞，有基因表达的次生细胞。每种细胞类型在其基因的表达上是一致的。我们的研究结果表明，这两种细胞类型不仅在形态上不同，而且在生物化学上也不同。我们还表明，这两种细胞类型不同的基因表达的调节交配活动。
The accessory gland of the male Drosophila melanogaster plays a vital role in reproduction. This secretory organ synthesizes products that are transferred to the female and are necessary to elicit the proper physiological and behavioral responses in the female. The accessory gland is composed of two morphologically distinct secretory cell types, the main cells and the secondary cells. Previous studies identified some genes expressed in main cells or in all accessory gland cells. In this paper we use P-element mediated enhancer traps to examine gene expression in the accessory gland. We show that, in addition to genes expressed in main cells only or in all accessory gland secretory cells, there are genes expressed specifically in secondary cells. Each cell type is uniform in the expression of its genes. Our results demonstrate that the two cell types are not only morphologically distinct but also biochemically distinct. We also show that the two cell types differ in their regulation of gene expression in response to mating activity.