CYTOSKELETON AND DIFFERENTIATION - EFFECTS OF CYTOCHALASIN B AND COLCHICINE ON EXPRESSION OF THE DIFFERENTIATED PHENOTYPE OF RABBIT COSTAL CHONDROCYTES IN CULTURE
CYTOSKELETON AND DIFFERENTIATION - EFFECTS OF CYTOCHALASIN B AND COLCHICINE ON EXPRESSION OF THE DIFFERENTIATED PHENOTYPE OF RABBIT COSTAL CHONDROCYTES IN CULTURE
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DOI：
10.1016/0045-6039(84)90046-0
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
SUZUKI, F
中科院分区：
文献类型：
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作者：
TAKIGAWA, M;TAKANO, T;SUZUKI, F
细胞松弛素B使培养的兔肋软骨细胞由多角形变为近球形，并刺激糖胺聚糖的合成，这是软骨细胞的分化表型，而秋水仙素使软骨细胞由多角形变为扁平，并抑制糖胺聚糖的合成。这些形态变化与糖胺聚糖合成的变化同时发生。甲状旁腺激素诱导的鸟氨酸脱羧酶（鸟氨酸脱羧酶是软骨细胞分化的良好标志）在细胞松弛素B预处理的球形细胞中明显增强，而秋水仙碱预处理则抑制了该酶的诱导。细胞松弛素B和秋水仙碱均抑制DNA合成。在细胞形态和糖胺聚糖合成发生变化后观察到抑制作用。微管的完整性和微丝的断裂参与调节培养软骨细胞分化表型的表达。
Cytochalasin B changed the shape of cultured rabbit costal chondrocytes from polygonal to nearly spherical and stimulated glycosaminoglycan synthesis, which is a differentiated phenotype of chondrocytes, whereas colchicine changed them from polygonal to flattened and inhibited glycosaminoglycan synthesis. These morphological changes occurred parallel with the changes in glycosaminoglycan synthesis. Induction of ornithine decarboxylase by parathyroid hormone, which is a good marker of differentiated chondrocytes, was markedly potentiated in the spherical cells which were pretreated with cytochalasin B, whereas pretreatment with colchicine inhibited the induction of the enzyme. Both cytochalasin B and colchicine inhibited DNA synthesis. The inhibitions were observed after the appearance of changes in the morphology of the cells and glycosaminoglycan synthesis. Intactness of microtubules and disruption of microfilaments are involved in regulating the expression of the differentiated phenotype of chondrocytes in culture.