The problem of cultivation of Mycobacterium leprae: a review with criteria for evaluating recent experimental work.
The problem of cultivation of Mycobacterium leprae: a review with criteria for evaluating recent experimental work.
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麻风分枝杆菌的培养问题：最近实验工作评估标准的综述。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
S. Pattyn
中科院分区：
文献类型：
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作者：
S. Pattyn
提出了一些标准，以帮助评估出现在文献中声称成功培养麻风分枝杆菌的论文，无论是在缺乏或存在组织培养的细胞。最近的电镜研究明确地表明麻风分枝杆菌属，并通过横切面进行了分裂。一项调查给出了分枝杆菌菌株分离在过去10年麻风病变。这些菌株在分类上属于不同的物种，不能认为是麻风分枝杆菌。关于麻风分枝杆菌体外生长没有实质性的声明，组织培养技术的应用也同样令人失望。作者认为，麻风分枝杆菌体外培养的进展只能通过应用现代生化和组织化学技术来增加对细胞内环境和该生物代谢活动的了解。
Some criteria are presented to help evaluate papers appearing in the literature claiming successful cultivation of M. leprae either in the absence or in the presence of tissue-cultured cells. Recently, electrom microscopic studies have definitely shown M. leprae to belong to the genus Mycobacterium and its division to occur through transverse section. A survey is given of the mycobacterial strains isolated in the last 10 years from leprosy lesions. These strains belong to taxonomically different species and cannot be considered to be M. leprae. No substantial claim was made concerning the in vitro growth of M. leprae and the application of the tissue culture technique has been equally disappointing. The view is expressed that progress towards the in vitro cultivation of M. leprae can be made only as a result of increased knowledge about the intracellular environment and the metabolic activities of this organism, to be obtained by the application of modern biochemical and histochemical techniques.