PCR based determination of mitochondrial DNA copy number in multiple species.
PCR based determination of mitochondrial DNA copy number in multiple species.
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DOI：
10.1007/978-1-4939-1875-1_3
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Meyer, Joel N
中科院分区：
文献类型：
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作者：
Rooney, John P;Ryde, Ian T;Sanders, Laurie H;Howlett, Evan H;Colton, Meryl D;Germ, Kaylyn E;Mayer, Greg D;Greenamyre, J Timothy;Meyer, Joel N
线粒体DNA(MtDNA)拷贝数是线粒体整体健康的重要组成部分。在这一章中，我们描述了分离线粒体DNA和核DNA(核DNA)的方法，并用定量聚合酶链式反应测量它们各自的拷贝数。根据起始材料的种类和细胞类型以及特定的PCR试剂的可用性，方法会有所不同。
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA), and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material, and availability of specific PCR reagents.