Vaccination of pigs two weeks before infection significantly reduces transmission of foot-and-mouth disease virus
Vaccination of pigs two weeks before infection significantly reduces transmission of foot-and-mouth disease virus
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DOI：
10.1016/j.vaccine.2003.11.003
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发表时间：
2004-03-29
期刊：
影响因子：
5.5
通讯作者：
Dekker, A
中科院分区：
文献类型：
--
作者：
Eblé, PL;Bouma, A;Dekker, A
本研究的目的是调查是否以及在什么时间间隔接种疫苗可以减少口蹄疫病毒（FMDV）在猪中的传播。通过接种疫苗减少病毒传播是通过实验确定的。在三组10头猪中研究了FMDV的传播：一个未接种组和两个分别在接种前7天（-7 dpi）和14天（-14 dpi）接种的组。从每组中随机选择5头猪接种FMDV O型台湾，而组中剩下的另外5头猪通过直接接触与接种的猪接触。记录临床症状，进行口咽液（OPF）病毒分离和RT-PCR，测定中和抗体滴度和抗FMDV非结构蛋白（NS）抗体反应。在-14 dpi组中未观察到病毒传播，而在影响未接种组和-7 dpi组的所有接触猪中均观察到病毒传播。-14dpi疫苗组的繁殖率R明显低于未疫苗组。这项研究证实了疫苗接种作为减少FMDV传播的重要工具的潜力。(C)2003 Elsevier Ltd.保留所有权利。
The objective of this study was to investigate whether and at what time interval could vaccination reduce transmission of foot-and-Mouth disease virus (FMDV) among pigs. Reduction of virus transmission by vaccination was determined experimentally. Transmission of FMDV was studied in three groups of ten pigs: one non-vaccinated group and two groups that were vaccinated 7 days (-7 dpi) and 14 days before inoculation (-14 dpi), respectively. Five randomly selected pigs from each group were inoculated with FMDV type O Taiwan, while the other five pigs left in the groups were exposed to the inoculated pigs by direct contact. Clinical signs were recorded, virus isolation and RT-PCR were carried out on oropharyngeal fluid (OPF), and the neutralizing antibody titres and the antibody response against non-structural (NS) proteins of FMDV were determined. No virus transmission was observed in the -14 dpi group, whereas virus transmission was observed in all contact pigs affecting both the non-vaccinated and the -7 dpi group. The reproduction ratio R in the -14 dpi vaccinated group was significantly lower than that of the non-vaccinated group. This study confirms the potential of vaccination as an important tool to reduce transmission of FMDV. (C) 2003 Elsevier Ltd. All rights reserved.