Quantitative analysis of human keratinocyte cell elasticity using atomic force microscopy (AFM).
Quantitative analysis of human keratinocyte cell elasticity using atomic force microscopy (AFM).
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DOI：
10.1109/tnb.2011.2113397
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发表时间：
2011-03
影响因子：
3.9
通讯作者：
Sinha AA
中科院分区：
文献类型：
--
作者：
Fung CK;Xi N;Yang R;Seiffert-Sinha K;Lai KW;Sinha AA
本文介绍了利用原子力显微镜（AFM）在纳米尺度下对生理和病理生理条件下上皮细胞连接相互作用的动态进行可视化和量化。桥粒连接是上皮组织内细胞黏附所必需的特殊结构。在诸如天疱疮等水疱性皮肤病中，由于针对特定桥粒蛋白的自身抗体的产生，可观察到这些连接的解体。然而，这些结构复杂且力学性质不均匀，这使得研究困难，并且自身抗体介导的角质形成细胞解体机制在很大程度上仍然未知。在此，我们利用原子力显微镜系统对培养的活体皮肤上皮细胞进行成像并测量其力学特性。我们证明力测量数据可能区分经不同抗体处理的细胞。我们对原子力显微镜用于原位成像和弹性测量的展示使我们能够更详细地开始研究水疱性皮肤病的疾病机制并监测治疗策略，以满足在局部或组织水平理解疾病病理学的需求。
This paper presents the use of atomic force microscopy (AFM) to visualize and quantify the dynamics of epithelial cell junction interactions under physiological and pathophysiological conditions at the nanoscale. Desmosomal junctions are specialized structures critical to cellular adhesion within epithelial tissues. Disassembly of these junctions is seen consequent to the development of autoantibodies directed at specific desmosomal proteins in blistering skin diseases such as Pemphigus. However, these structures are complex and mechanically inhomogeneous, making it difficult to study and the mechanisms of autoantibody mediated keratinocyte disassembly remain largely unknown. Here, we have used AFM system to image and measure the mechanical property of living skin epithelial cells in culture. We demonstrate that the force measurement data can possibly distinguish the cell with different antibody treatment. Our demonstration of the use of AFM for in situ imaging and elasticity measurement positioned us to begin to investigate disease mechanisms and monitor therapeutic strategies in blistering skin diseases in much greater detail, to meet the demands for understanding disease pathology at the local, or tissue level.