Electrochemical gene detection based on supramolecular complex formation by ferrocenyl-β-cyclodextrin and adamantylnaphthalene diimide bound to double stranded DNA
Electrochemical gene detection based on supramolecular complex formation by ferrocenyl-β-cyclodextrin and adamantylnaphthalene diimide bound to double stranded DNA
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DOI：
10.1016/j.jorganchem.2004.08.039
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发表时间：
2004-12-06
影响因子：
2.3
通讯作者：
Takenaka, S
中科院分区：
文献类型：
--
作者：
Sato, S;Nojima, T;Takenaka, S
合成了高选择性双链DNA （dsDNA）结合试剂金刚烷基萘二亚胺1。以超声小牛胸腺DNA作为dsDNA模型的结合研究表明，1与dsDNA可以通过螺纹方式结合，其中两个金刚烷基部分分别位于dsDNA的主槽和次槽中，并且通过β -环糊精对1与dsDNA结合的金刚烷基部分进行旋盖来稳定1与DNA双工的复合物。1的金刚烷基部分也可以结合到二茂铁- β -环糊精（fc - β - cd）的空腔中，从而形成超分子复合物。当该络合物在DNA探针固定电极上形成时，电化学DNA检测是可行的：与目标DNA杂交的电极在1和fc - β - cd处理后产生对应于二茂铁氧化的电流峰。(C) 2004 Elsevier B.V.版权所有
Adamantylnaphthalene diimide 1 was synthesized as a highly selective double stranded DNA (dsDNA) binding reagent. The binding studies with sonicated calf thymus DNA as a model of dsDNA revealed that 1 can bind to dsDNA by the threading mode, where the two adamantyl moieties are located in the major and minor grooves of dsDNA separately and the complex of 1 with DNA duplex was stabilized by capping of the adamantyl moieties of 1 bound to dsDNA by beta-cyclodextrin. The adamantyl moieties of 1 could be incorporated also into the cavity of ferrocenyl-beta-cyclodextrin (Fc-beta-CD), resulting in the formation of a supramolecular complex. When this complex is formed on the DNA probe-immobilized electrode, electrochemical DNA detection was feasible: the electrode hybridized with target DNA gave rise to a current peak corresponding to the ferrocene oxidation upon treatment with 1 and Fc-beta-CD. (C) 2004 Elsevier B.V. All rights reserved.