ISOLATION AND CHARACTERIZATION OF MUTATIONS AFFECTING TRANSPORT OF HEXOSE PHOSPHATES IN ESCHERICHIA-COLI
ISOLATION AND CHARACTERIZATION OF MUTATIONS AFFECTING TRANSPORT OF HEXOSE PHOSPHATES IN ESCHERICHIA-COLI
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DOI：
10.1128/jb.113.2.895-900.1973
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发表时间：
1973-01-01
影响因子：
3.2
通讯作者：
WINKLER, HH
中科院分区：
文献类型：
--
作者：
KADNER, RJ;WINKLER, HH
分离出感染磷酸己糖转运系统的大肠杆菌突变体。采用青霉素处理的负选择或磷霉素的正选择。这些突变体在磷酸己糖以外的所有碳源上正常生长。通过转导杂交研究了 18 个独立分离的突变株中突变的图谱位置。发现所有突变都位于染色体的同一区域，即泰勒图上第 72 分钟所代表的区域。发现该区域中基因的顺序为bemtl-cysE-pyrE-uhp-bgl-ilv。一些突变体的回复体表现出对该运输系统的调节控制的改变。
Mutants ofEscherichia colidefective in the hexose phosphate transport system were isolated. Negative selection by penicillin treatment or positive selection with phosphonomycin was employed. These mutants grew normally on all carbon sources other than hexose phosphates. The map location of the mutations in 18 independently isolated mutant strains was investigated by transduction crosses. All of the mutations were found to lie in the same region of the chromosome, in the region represented by min 72 on the Taylor map. The order of the genes in this region was found to bemtl-cysE-pyrE-uhp-bgl-ilv. Revertants of some of the mutants exhibited altered regulatory control of this transport system.