Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women
Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women
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DOI：
10.1194/jlr.m300256-jlr200
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发表时间：
2004-01-01
影响因子：
6.5
通讯作者：
Norstedt, G
中科院分区：
文献类型：
--
作者：
Linder, K;Arner, P;Norstedt, G
越来越多的证据表明，体内脂肪组织的分布对于肥胖代谢并发症（如糖尿病、高血压和高脂血症）的发生至关重要。本研究的目的是使用消减杂交策略来鉴定肥胖男性皮下和网膜人类脂肪组织中差异表达的基因。从获得的一组差异表达转录本中，我们还旨在鉴定在网膜或皮下脂肪组织中具有性别特异性表达模式的基因。对一名极度腹部肥胖男性的皮下和网膜脂肪组织的 cDNA 进行了代表性差异分析 (RDA)。鉴定出四十四个推定的差异表达基因。将获得的 RDA 产品点样到载玻片上，通过微阵列杂交程序筛选其他肥胖患者的差异表达。已证实有五个基因在男性或女性肥胖患者的皮下或网膜脂肪组织中存在差异表达。仅在男性中检测到一种基因，并且发现该基因在皮下组织中表达上调。这些发现扩展了先前的知识，即不同的脂肪库具有差异的基因表达，并表明脂肪基因表达模式存在性别差异。
There is growing evidence that the distribution of adipose tissue in the body is of importance in the development of metabolic complications of obesity, such as diabetes, hypertension, and hyperlipidemia. The aim of this study was to identify differentially expressed genes in subcutaneous and omental human adipose tissue in obese men, using a subtractive hybridization strategy. From the obtained set of differentially expressed transcripts, we also aimed to identify genes that have a sex-specific pattern of expression in omental or subcutaneous adipose tissue. Representational difference analysis (RDA) was performed on cDNA from subcutaneous and omental fat tissue from a man with extreme abdominal obesity. Forty-four putatively differentially expressed genes were identified. The obtained RDA products were spotted onto glass slides to screen for differential expression in other obese patients by using a microarray hybridization procedure. Five genes were confirmed to be differentially expressed in subcutaneous or omental adipose tissue from male or female obese patients. One gene was detected only in males and was found to be upregulated in subcutaneous tissue. The findings extend previous knowledge that different fat depots have differential gene expression and indicate that sex differences exist in adipose gene expression patterns.