Cytotoxicity of anti-Fab antibodies against B lymphocytes in rheumatoid arthritis.
Cytotoxicity of anti-Fab antibodies against B lymphocytes in rheumatoid arthritis.
复制标题
类风湿性关节炎中抗 Fab 抗体针对 B 淋巴细胞的细胞毒性。
DOI：
10.1002/art.1780241007
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发表时间：
1981
影响因子：
--
通讯作者：
Terasaki,PI
中科院分区：
文献类型：
--
作者：
Nasu,H;Chia,D;Iwaki,Y;Barnett,EV;Terasaki,PI
70% 的类风湿性关节炎患者（77 名中的 54 名）和 3% 的健康捐献者（35 名中的 1 名）抗 Fab 抗体滴度呈阳性。通过抑制抗 Fab 抗体的放射免疫测定来检查它们的特异性，证明它们针对完整免疫球蛋白分子的 Fd 区域。此外，抗 Fab 抗体对 IgG 抗原具有广泛的特异性，骨髓瘤 IgG 的抑制作用表明了这一点。它们对 B 细胞（来自大多数健康捐献者的 20% 的 B 细胞）具有细胞毒性，但对 T 淋巴细胞没有细胞毒性，5°C 时的细胞毒性高于 37°C。这些研究表明，抗 Fab 抗体与 B 淋巴细胞表面抗原亚群的同种异型或独特型决定簇相互作用。
Anti‐Fab antibody titers were positive in 70% of rheumatoid arthritis patients (54 of 77) and 3% (1 of 35) of healthy donors. Their specificity was examined by inhibition of the radioimmunoassay for anti‐Fab antibodies, which demonstrated that they are against the Fd region of the intact immunoglobulin molecule. In addition, anti‐Fab antibodies have broad specificities for IgG antigens, shown by inhibition with myeloma IgG. They are cytotoxic against B (20% of B cells from most healthy donors) but not T lymphocytes, with cytotoxicity greater at 5°C than 37°C. These studies show that the anti‐Fab antibodies interact with allotypic or idiotypic determinants on subpopulations of B lymphocyte cell surface antigens.