Baicalin improves the in vitro developmental capacity of pig embryos by inhibiting apoptosis, regulating mitochondrial activity and activating sonic hedgehog signaling
Baicalin improves the in vitro developmental capacity of pig embryos by inhibiting apoptosis, regulating mitochondrial activity and activating sonic hedgehog signaling
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黄芩苷通过抑制细胞凋亡、调节线粒体活性和激活声刺猬信号来提高猪胚胎的体外发育能力
DOI：
10.1093/molehr/gaz036
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发表时间：
2019-09-01
影响因子：
4
通讯作者：
Kang，Jin-Dan
中科院分区：
文献类型：
--
作者：
Guo，Qing;Xuan，Mei-Fu;Kang，Jin-Dan
黄芩苷是一种化学结构已知的中药单体，可用于治疗女性不孕症。然而，黄芩苷对胚胎发育的影响尚不清楚。本研究探讨了黄芩甙对猪孤雌激活胚胎（PA）和体外受精胚胎（IVF）体外发育的影响及其可能机制。0.1 μg/ml黄芩苷能显著提高PA猪胚胎的体外发育能力（P< 0.05），降低活性氧（ROS）水平和细胞凋亡，提高线粒体膜电位（Δ Km）和ATP水平。黄芩苷处理组2-细胞期PA猪胚胎中与SHH信号通路相关的Sonic Hedgehog（SHH）和GLI 1的mRNA和蛋白表达显著高于对照组。为了证实SHH信号通路参与黄芩苷改善胚胎发育的机制，我们在不存在或存在环巴胺（Cy）（该通路的抑制剂）的情况下用黄芩苷处理胚胎。环磷酰胺能消除黄芩苷对体外胚胎发育的影响。结论黄芩苷通过抑制ROS产生、抑制细胞凋亡、调节线粒体活性、激活SHH信号通路，提高PA和IVF猪胚胎的体外发育能力。
Baicalin, a traditional Chinese medicinal monomer whose chemical structure is known, can be used to treat female infertility. However, the effect of baicalin on embryonic development is unknown. This study investigated the effects of baicalin onin vitrodevelopment of parthenogenetically activated (PA) andin vitrofertilized (IVF) pig embryos and the underlying mechanisms involved. Treatment with 0.1 μg/ml baicalin significantly improved (P< 0.05) thein vitrodevelopmental capacity of PA pig embryos by reducing the reactive oxygen species (ROS) levels and apoptosis and increasing the mitochondrial membrane potential (ΔΨm) and ATP level. mRNA and protein expression of sonic hedgehog (SHH) and GLI1, which are related to the SHH signaling pathway, in PA pig embryos at the 2-cell stage, were significantly higher in the baicalin-treated group than in the control group. To confirm that the SHH signaling pathway is involved in the mechanism by which baicalin improves embryonic development, we treated embryos with baicalin in the absence or presence of cyclopamine (Cy), an inhibitor of this pathway. Cy abolished the effects of baicalin onin vitroembryonic development. In conclusion, baicalin improves thein vitrodevelopmental capacity of PA and IVF pig embryos by inhibiting ROS production and apoptosis, regulating mitochondrial activity and activating SHH signaling.