Hematopoietic stem cells engraft in mice with absolute efficiency
Hematopoietic stem cells engraft in mice with absolute efficiency
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DOI：
10.1038/ni940
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发表时间：
2003-07-01
影响因子：
30.5
通讯作者：
Iscove, NN
中科院分区：
文献类型：
--
作者：
Benveniste, P;Cantin, C;Iscove, NN
小鼠造血干细胞（hsc）的植入被认为是一个低效的过程，但尚未被直接测量。我们使用了两种独立的策略来测试它们的移植效率：一种是测量未纯化的供体骨髓细胞与受体细胞在小鼠宿主中的竞争，另一种是跟踪每个受体注射一个高度纯化的干细胞的移植。结果表明，干细胞的移植几乎具有绝对的效率。因此，低效率的移植不能解释纯HSC部分所观察到的永久性重构的低频率，而是表明大多数最初移植的细胞不能维持自我更新。
The engraftment of murine hematopoietic stem cells (HSCs) into irradiated mice is thought to be an inefficient process, but has yet to be measured directly. We used two independent strategies to test their engraftment efficiency: one measured competition of unpurified donor bone marrow cells with recipient cells in murine hosts and the other tracked the engraftment of one highly purified stem cell injected per recipient. The results showed that stem cells engrafted with near absolute efficiency. Thus, inefficient engraftment cannot explain the low frequency of permanent reconstitutions observed with pure HSC fractions and instead suggests most initially engrafted cells fail to sustain self-renewal.