Basic-helix-loop-helix transcription factor DEC2 constitutes negative feedback loop in IFN-β-mediated inflammatory responses in human mesangial cells
Basic-helix-loop-helix transcription factor DEC2 constitutes negative feedback loop in IFN-β-mediated inflammatory responses in human mesangial cells
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DOI：
10.1016/j.imlet.2010.11.009
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发表时间：
2011-04-30
影响因子：
4.4
通讯作者：
Satoh, Kei
中科院分区：
文献类型：
--
作者：
Imaizumi, Tadaatsu;Sato, Fuyuki;Satoh, Kei
分化胚胎-软骨细胞2 （Differentiated embryo-chondrocyte 2, DEC2）是一种碱性螺旋-环-螺旋转录因子，通过调控其靶基因的表达参与多种生物反应。在本研究中，我们在培养的人系膜细胞中证实了DEC2在多肌苷-多胞酸（poly IC）（一种真正的双链RNA）处理下的表达。RNA干扰DEC2增强了聚ic诱导的ifn - β及其下游基因、视黄酸诱导基因-i （RIG-I）和CCL5的表达。敲低TLR3可消除poly - ic诱导的DEC2表达。DEC2的表达可能构成肾小球内ifn - β /RIG-I/CCL5通路的负反馈系统，可能在控制肾脏的持续性炎症反应中发挥作用。(C) 2010 Elsevier B.V.版权所有
Differentiated embryo-chondrocyte 2 (DEC2), a basic-helix-loop-helix transcriptional factor, is involved in various biological reactions by regulating the expression of its target genes. In the present study, we demonstrated DEC2 expression in response to the treatment with polyinosinic-polycytidylic acid (poly IC), an authentic double-stranded RNA, in cultured human mesangial cells. RNA interference against DEC2 enhanced the poly IC-induced expression of IFN-beta and its downstream genes, retinoic acid-inducible gene-I (RIG-I) and CCL5. Knockdown of TLR3 abolished the poly IC-induced DEC2 expression. DEC2 expression may constitute a negative feedback system for the IFN-beta/RIG-I/CCL5 pathway in the glomerulus, which may play a role in controlling protracted inflammatory reactions in the kidney. (C) 2010 Elsevier B.V. All rights reserved.