Campylobacter jejuni infection in broiler chickens
Campylobacter jejuni infection in broiler chickens
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DOI：
10.1637/7411-071405r.1
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发表时间：
2006-03-01
期刊：
影响因子：
1.4
通讯作者：
Bandli, D
中科院分区：
文献类型：
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作者：
Dhillon, AS;Shivaprasad, HL;Bandli, D
从位于太平洋西北部的孵化场采购一日龄直跑肉鸡。将鸡单独细分为9组，每组20只鸡。将鸡标记，圈养在铁丝隔离室中，饲喂商业肉鸡饲料，并随意饮水。本研究检测了3株家禽源空肠弯曲菌分离株和1株人源空肠弯曲菌分离株。各种C.通过作物管饲法将空肠培养物接种到9日龄鸡中。以0.5 ml 1 x 10（2）菌落形成单位（CFU）/ml的剂量水平接种4组20只鸡。其他四组接种0.5 ml 1 x 10（4）CFU/ml。一组20只鸡作为未接种对照组。在接种后5、12和19天（DPI），从接种组和未接种组中随机选择4只鸡进行尸检。培养空肠弯曲菌，并从上肠、中肠和盲肠的复合物中计数。所有鸡组的体重。7日龄和5、12和19 DPI时测量并进行统计学分析。与未接种对照组相比，在两个剂量水平下接种空肠弯曲菌的7日龄、5 DPI和12 DPI雄性和雌性肉鸡的平均体重（MBW）无显著差异。在19 DPI时，在某些组中观察到雄性和雌性肉鸡的MBW差异。所有接种鸡组在5 DPI时复合肠样本的空肠弯曲菌培养计数平均值（CEM）结果（不考虑剂量水平）范围为（2.5 +/- 5.0）x 10（2）至（2.8 +/- 4.8）x 10（5）CFU/g（平均值+/- SD）。在所有处理组中，无论剂量水平如何，在两个剂量水平下接种的5 DPI时盲肠空肠弯曲菌CEM的结果范围为（2.5 +/- 5.0）x 10（6）至（1 +/- 0.0）x 10（7）CFU/g。在12和19 DPI时来自复合肠样品的CEM结果增加了1个对数单位，或有时更多。盲肠G.在所有处理组中，以两种剂量水平接种的5 DPI空肠CEM的范围为（2.5 +/- 5.0）x 10（6）至（1 +/- 0.0）x 10（7）CFU/g，与剂量水平无关。在12 DPI时，在接种1 × 10（2）CFU空肠弯曲菌的鸡组中，空肠弯曲菌CEM增加2-5 log单位，在接种较高剂量水平空肠弯曲菌的鸡组中，增加2- 3 log单位。在19 DPI时来自盲肠样品的空肠弯曲菌CEM的结果与在12 DPI时鸡组的结果相似。在5、12和19 DPI时未从未接种的对照鸡中分离出空肠弯曲菌。本研究期间，任何鸡组均未出现疾病或大体病理学病变的临床体征。在接种空肠弯曲菌的鸡或未接种的对照鸡中，组织病理学评价中不存在病变。
Day-old, straight-run broiler chickens were procured from a hatchery located in the Pacific Northwest. The chickens were Subdivided individually into nine groups of 20 chickens. The chickens were tagged, housed in isolation chambers on wire, fed commercial broiler feed, and given water ad libitum. Three isolates of Campylobacter jejuni of poultry origin and one of human origin were tested in this Study. Various C. jejuni cultures were inoculated into 9-day-old chickens by crop gavage. Four groups of 20 chickens were inoculated at a dose level of 0.5 ml of 1 x 10(2) colony-forming units (CFU)/ml. The other four groups were inoculated with 0.5 ml of 1 x 10(4) CFU/mI. One group of 20 chickens was kept as an uninoculated control group. Four randomly selected chickens from each of the inoculated and uninoculated groups Were necropsied at 5, 12, and 19 days postinoculation (DPI). The C.jejuni was cultured and enumerated from a composite of the upper and midintestine and the cecum. Body weights of all chicken groups at. 7 days of age and at 5, 12, and 19 DPI were measured and statistically analyzed. No significant differences were present in the mean body weights (MBWs) of 7-day-old, 5 DPI, and 12 DPI male and female broiler chickens inoculated with C jejuni at both dose levels compared with uninoculated controls. Differences in MBWs of the male and female broilers at 19 DPI were observed in some of the groups. Results of the C jejuni culture enumeration mean (CEM) OF composite intestine samples at 5 DPI from all inoculated chicken groups, irrespective of the dose level, ranged from (2.5 +/- 5.0) x 10(2) to (2.8 +/- 4.8) x 10(5) CFU/g (mean +/- SD). Results oF cecum C.jejuni CEM at 5 DPI inoculated at both dose levels ranged from (2.5 +/- 5.0) x 10(6) to (1 +/- 0.0) x 10(7) CFU/g in all treatment groups irrespective of the dose level. CEM results from the composite intestine samples at 12 and 19 DPI increased by 1 log Unit, or sometimes more. Results of cecum G. jejuni CEM at 5 DPI inoculated at both dose levels ranged from (2.5 +/- 5.0) x 10(6) to (1 +/- 0.0) x 10(7) CFU/g in all treatment groups irrespective of the dose level. Increases of 2-5 log units in C.jejuni CEM was present in chicken groups inoculated with 1 x 10(2) CFU of C.jejuni, and a 2- to 3-log increase was present in groups inoculated with a higher dose level of C.jejuni at 12 DPI. The results of C jejuni CEM from cecal samples at 19 DPI were similar to chicken groups at 12 DPI Campylobacter jejuni was not isolated from the uninoculated control chickens at 5, 12, and 19 DPI. Clinical signs of illness or gross pathologic lesions were not present in any of the chicken groups during this Study. No lesions were present on histopathologic evaluations in C.jejuni-inoculated chickens or uninoculated control chickens.