Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants
Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants
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DOI：
10.1016/j.plantsci.2015.03.020
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发表时间：
2015-07-01
期刊：
影响因子：
5.2
通讯作者：
Kim, Woo Taek
中科院分区：
文献类型：
--
作者：
Byun, Mi Young;Lee, Jungeun;Kim, Woo Taek
金鱼草是一种生长在南极半岛西海岸的南极毛草。在本报告中，我们已经鉴定并鉴定了一个转录因子--南极洲D.C-重复结合因子7(DaCBF7)，它是单子叶植物V CBF同源物的成员。该蛋白含有一个单一的AP2结构域、一个假定的核定位信号和典型的CBF特征。DaCBF7与其他单子叶植物V组同源物一样，在AP2基序前面含有一个由43个氨基酸组成的独特的多肽延伸部分。DaCBF7主要定位于细胞核，并在体外与C-重复/脱水反应元件(CRT/DRE)核心序列(ACCGAC)相互作用。DaCBF7是由干旱、寒冷和盐度等非生物胁迫诱导的。为了研究其在耐冷性中可能的细胞作用，采用了转基因水稻系统。与无生长缺陷的野生型水稻相比，转DaCBF7基因水稻植株(Ubi：DaCBF7)对低温胁迫的耐受性显著提高，但对干旱或盐胁迫的耐性影响不大。Ubi：DaCBF7转基因株系的转录组分析显示，在没有冷胁迫和短期或长期冷胁迫下，DaCBF7过表达的植株与野生型植株相比上调了13个基因。其中5个基因，即脱水素、雷莫林、Os03g63870、Os11g34790和Os10g22630，在其启动子区域含有可能的CRT/DRE或低温反应元件。这些结果表明，DaCBF7的过表达直接和间接地诱导了转基因水稻植株中不同的基因，并增强了水稻对低温胁迫的耐受性。(C)2015爱思唯尔爱尔兰有限公司。保留所有权利。
Deschampsia antarcdca is an Antarctic hairgrass that grows on the west coast of the Antarctic peninsula. In this report, we have identified and characterized a transcription factor, D. antarctica C-repeat binding factor 7 (DaCBF7), that is a member of the monocot group V CBF homologs. The protein contains a single AP2 domain, a putative nuclear localization signal, and the typical CBF signature. DaCBF7, like other monocot group V homologs, contains a distinct polypeptide stretch composed of 43 amino acids in front of the AP2 motif. DaCBF7 was predominantly localized to nuclei and interacted with the C-repeat/dehydration responsive element (CRT/DRE) core sequence (ACCGAC) in vitro. DaCBF7 was induced by abiotic stresses, including drought, cold, and salinity. To investigate its possible cellular role in cold tolerance, a transgenic rice system was employed. DaCBF7-overexpressing transgenic rice plants (Ubi:DaCBF7) exhibited markedly increased tolerance to cold stress compared to wild-type plants without growth defects; however, overexpression of DaCBF7 exerted little effect on tolerance to drought or salt stress. Transcriptome analysis of a Ubi:DaCBF7 transgenic line revealed 13 genes that were up-regulated in DaCBF7-overexpressing plants compared to wild-type plants in the absence of cold stress and in short- or long-term cold stress. Five of these genes, dehydrin, remorin, Os03g63870, Os11g34790, and Os10g22630, contained putative CRT/DRE or low-temperature responsive elements in their promoter regions. These results suggest that overexpression of DaCBF7 directly and indirectly induces diverse genes in transgenic rice plants and confers enhanced tolerance to cold stress. (C) 2015 Elsevier Ireland Ltd. All rights reserved.