ROLE OF MACROPHAGE MIGRATION INHIBITORY FACTOR IN AGE-RELATED HEARING LOSS
ROLE OF MACROPHAGE MIGRATION INHIBITORY FACTOR IN AGE-RELATED HEARING LOSS
复制标题
DOI：
10.1016/j.neuroscience.2014.08.042
复制
发表时间：
2014-10-24
期刊：
影响因子：
3.3
通讯作者：
Nishizaki, K.
中科院分区：
文献类型：
--
作者：
Kariya, S.;Okano, M.;Nishizaki, K.
与衰老相关的听力损失是老年人中最常见的感觉障碍。巨噬细胞迁移抑制因子（MIF）是一种多功能分子。本研究的目的是确定 MIF 在内耳中的作用。本研究使用BALB/c背景的MIF缺陷型小鼠（MIF-/-小鼠）和野生型BALB/c小鼠。通过免疫组织化学检查野生型小鼠 (WT) 内耳中 MIF 蛋白的表达。在 1、3、6、9、12 和 18 个月龄的 MIF-/- 小鼠和 WT 小鼠中，通过点击诱发的听觉脑干反应来评估听力功能。还使用扫描电子显微镜和光学显微镜对耳蜗进行了形态学检查。 MIF 在螺旋韧带、血管纹、赖斯纳膜、螺旋神经节细胞 (SGC)、囊状黄斑和膜迷路中观察到。与 WT 小鼠相比，MIF-/- 小鼠在 9、12 和 18 个月龄时听力损失显着。在MIF-/-小鼠中，扫描电子显微镜显示外耳蜗毛细胞受到影响，但内耳蜗毛细胞保存相对完好。 MIF-/- 小鼠的 SGC 数量较低。 MIF 在小鼠内耳中强烈表达。老年 MIF-/- 小鼠表现出与年龄相关的听力损失加速和内耳形态异常。这些发现表明 MIF 在小鼠内耳中发挥着重要作用。 (c) 2014 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
Hearing loss related to aging is the most common sensory disorder among elderly individuals. Macrophage migration inhibitory factor (MIF) is a multi-functional molecule. The aim of this study was to identify the role of MIF in the inner ear. MIF-deficient mice (MIF-/- mice) of BALB/c background and wild-type BALB/c mice were used in this study. Expression of MIF protein in the inner ear was examined by immunohistochemistry in wild-type mice (WT). The hearing function was assessed by the click-evoked auditory brain-stem response in both MIF-/- mice and WT at 1, 3, 6, 9, 12, and 18 months of age. Morphological examination of the cochlea was also performed using scanning electron microscopy and light microscopy. MIF was observed in the spiral ligament, stria vascularis, Reissner's membrane, spiral ganglion cells (SGCs), saccular macula, and membranous labyrinth. The MIF-/- mice had a significant hearing loss as compared with the WT at 9, 12, and 18 months of age. In the MIF-/- mice, scanning electron microscopy showed that the outer cochlear hair cells were affected, but that the inner cochlear hair cells were relatively well preserved. The number of SGCs was lower in the MIF-/- mice. MIF was strongly expressed in the mouse inner ear. Older MIF-/- mice showed accelerated age-related hearing loss and morphological inner ear abnormalities. These findings suggest that MIF plays an important role in the inner ear of mice. (c) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.