Accelerated transfer of cholesteryl esters in dyslipidemic plasma. Role of cholesteryl ester transfer protein.
Accelerated transfer of cholesteryl esters in dyslipidemic plasma. Role of cholesteryl ester transfer protein.
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加速胆固醇酯在血脂异常血浆中的转移。
DOI：
10.1172/jci112940
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Denke,M
中科院分区：
文献类型：
--
作者：
Tall,A;Granot,E;Brocia,R;Tabas,I;Hesler,C;Williams,K;Denke,M
在高密度脂蛋白（HDL）中合成的血浆胆固醇酯可以通过胆固醇酯转移蛋白（CETP）转移到其他脂蛋白。我们发现，与对照组相比，在孵育的高胆固醇血症兔血浆中，胆固醇酯从HDL到含载脂蛋白B的脂蛋白的传质增加了两倍。在高胆固醇血症血浆的同位素测定中，CETP的活性增加了2 - 4倍。从高胆固醇血症血浆中分离出数量增加的CETP样分子。与血脂正常的对照组相比，4例β-脂蛋白异常受试者的孵育血浆也显示胆固醇酯传质增加（3倍）。在整个或无脂蛋白的血浆中测定，CETP的活性增加了两倍。因此，在血脂异常的家兔和人血浆中，胆固醇酯从HDL转移到可能致动脉粥样硬化的含载脂蛋白B的脂蛋白增加。增强的转移部分是由于CETP活性的增加，可能反映了CETP质量的增加。图片
Plasma cholesteryl esters, synthesized in the high density lipoproteins (HDL), may be transferred to other lipoproteins by a cholesteryl ester transfer protein (CETP). We found a twofold increase in mass transfer of cholesteryl ester from HDL to apoB-containing lipoproteins in incubated hypercholesterolemic rabbit plasma compared with control. There was a two- to fourfold increase in the activity of CETP, measured in an isotopic assay in hypercholesterolemic plasma. A CETP-like molecule was isolated in increased amounts from hypercholesterolemic plasma. Incubated plasma from four dysbetalipoproteinemic subjects also showed an increase (threefold) in cholesteryl ester mass transfer, compared with normolipidemic controls. There was a twofold increase in the activity of CETP, assayed in whole or lipoprotein-free plasma. Thus, there is increased transfer of cholesteryl esters from HDL to potentially atherogenic apoB-containing lipoproteins in dyslipidemic rabbit and human plasma. The enhanced transfer results in part from increased activity of CETP, possibly reflecting an increase in CETP mass.Images