Phylogenetic analysis of algicidal bacteria (family Flavobacteriaceae) and selective detection by PCR using a specific primer set
Phylogenetic analysis of algicidal bacteria (family Flavobacteriaceae) and selective detection by PCR using a specific primer set
复制标题
杀藻细菌（黄杆菌科）的系统发育分析和使用特定引物组的 PCR 选择性检测
DOI：
10.2331/fishsci.65.432
复制
发表时间：
1999
影响因子：
1.9
通讯作者：
S. Hiroishi
中科院分区：
文献类型：
--
作者：
R. Kondo;I. Imai;K. Fukami;A. Minami;S. Hiroishi
对黄杆菌科杀藻类Chattonella-haga sp.的16S rDNA进行了测序和系统发育分析。J18/MOI菌株对细胞吞噬的杀灭作用菌株aa8-2、aa8-3和裸子藻生长抑制黄杆菌。菌株5N-3。这些杀藻菌株在系统发育上聚为海洋细胞吞噬/黄杆菌/类杆菌(CFB)组。黄杆菌。5N-3在系统发育上与伯顿冷蛇属聚为一类，而与杀藻滑行细菌不同。5N-3菌株与伯顿拟青霉的序列相似性仅为92%。菌株J18/M01和aa8-2(aa8-3)具有较高的序列相似性，达98.5%。尽管寄主藻类不同，它们的地理位置和分离年份也不同，但它们在系统发育上位于同一末端分支。用一组J18/MO1特异性引物对J18/M01菌株的16S rDNA进行聚合酶链式反应，检测到J18/M01菌株，表明16S rDNA中的J18/M01特异性序列可用于对J18/M01菌株的选择性检测。
Sequencing the 16S rDNA and phylogenetic analysis were performed on algicidal bacteria belong ing to the family Flavobacteriaceae, Chattonella-killing Cytophaga sp. strain J18/MOI, Heterocapsa killing Cytophaga sp. strains AA8-2 and AA8-3, and Gymnodinium growth-inhibiting Flavobacterium sp. strain 5N-3. These algicidal strains were clustered phylogenetically within the marine Cytophaga/Flavobacter/Bacteroides (CFB) group. Flavobacterium sp. 5N-3 was phylogenetically clustered with Psychroserpens burtonensis but not with the algicidal gliding bacteria. The sequence simi larity between strain 5N-3 and P. burtonensis was only 92%. The strains J18/M01 and AA8-2 (AA8-3) exhibited the high level of sequence similarity of 98.5%. Despite the differences of host algae and of lo cality and year of isolation, these algicidal bacteria were phylogenetically located on the same terminal branch. The PCR amplification of the 16S rDNA of the CFB strains using a single set of J18/MO1-specific primers resulted in specific detection of the J18/M01, indicating that the J18/M01-specific sequence in the 16S rDNA is useful for selective detection of the strain J18/M01.