Temporal profile of connexin 43 expression after photothrombotic lesion in rat brain
Temporal profile of connexin 43 expression after photothrombotic lesion in rat brain
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DOI：
10.1016/j.neuroscience.2006.09.054
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发表时间：
2007-01-19
期刊：
影响因子：
3.3
通讯作者：
Frahm, C.
中科院分区：
文献类型：
--
作者：
Haupt, C.;Witte, O. W.;Frahm, C.
局灶性缺血性损伤后，有几种机制导致受累区域的继发性扩张，因此增加了初始损伤。我们深入研究了大鼠脑内光血栓形成后星形胶质细胞连接蛋白43（Cx43）的表达。Cx43 mRNA和蛋白表达的时间分布进行了研究，在远程，结构未受伤的皮质和海马区。海马结构显示Cx43 mRNA阳性星形胶质细胞的数量增加和上调的蛋白质表达只在同侧层oriens。我们假设这个区域参与胶质瘢痕的形成。虽然Cx43 mRNA阳性细胞短暂增加，但损伤半球体感皮质的免疫反应性降低。观察到缺血后大脑皮层中Cx43蛋白的减少意味着间隙连接细胞间通讯的损伤，这可能对大脑有害。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Following focal ischemic injury, several mechanisms lead to secondary expansion of the affected area and therefore increase the initial damage. We thoroughly investigated the expression of astrocytic connexin 43 (Cx43) after photothrombosis in rat brain. The temporal profile of Cx43 mRNA as well as protein expression was studied in remote, structurally uninjured cortical and hippocampal areas. The hippocampal formation revealed an increased number of Cx43 mRNA positive astrocytes and an up-regulated protein expression exclusively in the ipsilateral stratum oriens. We assume a participation of this region in glia scar formation. While Cx43 mRNA positive cells were transiently increased, immunoreactivity was reduced in the somatosensory cortex of injured hemispheres. The observed decrease of Cx43 protein in the post-ischemic cerebral cortex implies an impairment of gap junctional intercellular communication which might be detrimental to the brain. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.