Peptide variability exists within alpha and beta subunits of the T cell receptor for antigen.
Peptide variability exists within alpha and beta subunits of the T cell receptor for antigen.
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抗原T细胞受体的Alpha和β亚基内存在肽变异性。
DOI：
10.1084/jem.158.4.1368
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发表时间：
1983-10-01
影响因子：
15.3
通讯作者：
Reinherz, E L
中科院分区：
文献类型：
--
作者：
Acuto, O;Meuer, S C;Hodgdon, J C;Schlossman, S F;Reinherz, E L
抗原的T细胞受体（Ti）最近被鉴定为由一个49-51- kdalton α和一个43-kdalton β亚基组成的90-kdalton T3相关克隆型结构，所述亚基是二硫键连接的。在这里，Ti分子从两个同种异体反应性CTL克隆衍生自相同的供体，但不同的特异性（CT 8 III和CT 4 II）直接比较后，与抗克隆型单克隆抗体分离。等电聚焦显示α亚基（pI 4.4-4.7）比β亚基（pI 6.0- 6.2）酸性更强，但每种糖蛋白种类是不同的。更重要的是，125 I标记的表面受体的二维肽图表明，Ti 1和Ti 2的β链似乎是独特的，只有两个共同的肽。相比之下，Ti 1和Ti 2 α链的肽图谱虽然不完全相同，但相关性更高。这些结果表明，人T细胞受体是由恒定区以及可变区，后者至少有一个是位于β亚基。
The T cell receptor for antigen (Ti) has recently been identified as a 90-kdalton T3-associated clonotypic structure composed of one 49-51- kdalton alpha and one 43-kdalton beta subunit, which are disulfide linked. Here, Ti molecules from two alloreactive CTL clones derived from the same donor but of differing specificities (CT8III and CT4II) are directly compared following isolation with anticlonotypic monoclonal antibodies. Isoelectric focusing shows that the alpha subunits (pI 4.4-4.7) are more acidic than the beta subunits (pI 6.0- 6.2) but that each glycoprotein species is distinctive. More importantly, two-dimensional peptide maps of 125I-labeled surface receptors indicate that the beta chains of Ti1 and Ti2 appear unique and share only two peptides in common. In contrast, peptide maps of Ti1 and Ti2 alpha chains are more related although not identical. These results suggest that the human T cell receptor is composed of constant as well as variable regions and that at least one of the latter is located within the beta subunit.