THE BIOSYNTHETIC-PATHWAY OF MHC CLASS-II BUT NOT CLASS-I MOLECULES INTERSECTS THE ENDOCYTIC ROUTE
THE BIOSYNTHETIC-PATHWAY OF MHC CLASS-II BUT NOT CLASS-I MOLECULES INTERSECTS THE ENDOCYTIC ROUTE
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DOI：
10.1016/0092-8674(90)90224-3
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发表时间：
1990-04-06
期刊：
影响因子：
64.5
通讯作者：
PLOEGH, HL
中科院分区：
文献类型：
--
作者：
NEEFJES, JJ;STOLLORZ, V;PLOEGH, HL
我们研究了细胞内交通和亚细胞分布的MHC I类和II类抗原的回收表面糖蛋白，转铁蛋白受体（TFR），在人类淋巴母细胞系JY相比。I类分子未检测到内化。II类分子被内化，但不循环。相反，Tfr被发现内化和再循环。II类分子的生物合成途径与I类分子的不同之处在于，它显示出从transGolgi到细胞表面的运输延迟（1-3小时）：这里它与内吞途径相交。使用抗MHC抗体的免疫电子显微镜显示存在强烈标记为II类分子的囊泡结构。有人提出，在这个网站的组合II类分子与加工抗原可能发生。
We studied the intracellular traffic and subcellular distribution of MHC class I and class II antigens in comparison with a recycling surface glycoprotein, the transferrin receptor (Tfr), in the human lymphoblastoid cell line JY. No internalization was detectable for class I molecules. Class II molecules were internalized but did not recycle. In contrast, Tfr was found to internalize and recycle. The biosynthetic pathway of class II molecules differs from that of class I molecules in that it shows a delay (1-3 hr) in transport from trans Golgi to cell surface: here it intersects the endocytic route. Immunoelectron microscopy using anti-MHC antibodies revealed the existence of vesicular structures that were intensely labeled for class II molecules. It is proposed that at this site combination of class II molecules with processed antigen could occur.