DYNAMIC MONITORING OF CORNEAL CARBOHYDRATE-METABOLISM USING HIGH-RESOLUTION DEUTERIUM NMR-SPECTROSCOPY
DYNAMIC MONITORING OF CORNEAL CARBOHYDRATE-METABOLISM USING HIGH-RESOLUTION DEUTERIUM NMR-SPECTROSCOPY
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DOI：
10.1016/0014-4835(88)90016-4
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发表时间：
1988-08-01
影响因子：
3.4
通讯作者：
CHENG, HM
中科院分区：
文献类型：
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作者：
AGUAYO, JB;MCLENNAN, IJ;CHENG, HM
用氘（D或2 H）NMR光谱监测兔角膜中的葡萄糖代谢。将角膜在5.5 mM氘代葡萄糖（葡萄糖-6，6-D2）中孵育。通过NMR方法可以检测到乳酸的2.5 μ g变化和葡萄糖的4.1 μ g变化。在完整的兔角膜中葡萄糖利用和乳酸产生的平均速率分别为248和151 μ g h-1。乳酸盐产生/葡萄糖利用率为0-60，即总葡萄糖的60%被代谢为乳酸盐，证实糖酵解是葡萄糖代谢的主要途径。此外，基于HDO信号的富集（其是指由水中的氘核产生的天然丰富的氘信号），通过克雷布斯循环及其相关途径的葡萄糖氧化估计为90 μ g h-1或总消耗的36%。氘NMR光谱学相对于其他NMR技术（例如13 C光谱学）的显著优点是：（1）由于氘代代谢物的弛豫时间短，采集时间更短;（2）HDO信号可以用作内部参考;以及（3）2 H标记化合物的成本显著降低和高可用性。因此，氘核磁共振光谱法是一种可靠和有效的手段，与角膜糖酵解活性移植前可以很容易地评估。
Glucose metabolism in rabbit corneas was monitored with deuterium (D or 2H) NMR spectroscopy. The corneas were incubated in 5.5 mM deuterated glucose (glucose-6, 6-D2). A 2.5 .mu.g change in lactate and a 4.1 .mu.g change in glucose could be detected by the NMR method. The mean rates of glucose utilization and lactate poduction in intact rabbit corneas were 248 and 151 .mu.g h-1, respectively. The lactate production/glucose utilization ratio of 0-60, i.e. 60% of total glucose dis metabolized to lactate, confirms that glycolysis is the principal pathway for glucose catabolism. Further, based on enrichment of the HDO signal (which refers to the naturally abundant deuterium signal arising from deuterons in water), glucose oxidation through Krebs cycle and its associated pathways is estimated to be 90.mu.g h-1 or 36% of total consumption. The significant advantages of deuterium NMR spectroscopy over other NMR techniques (e.g. 13C spectroscopy) are: (1) shorter acquisition times because of the short relaxation times of deuterated metabolites; (2) the HDO signal can be used as the internal reference; and (3) significant reduction in cost and high availability of 2H-labeled compounds. Deuterium NMR spectroscopy is therefore a reliable and effective means with which the corneal glycotyltic activity prior to transplantation can be readily assessed.