An Immunocytochemical Technique Offering Increased Sensitivity and Lowered Cost with a Streptavidin-Horseradish Peroxidase Conjugate
An Immunocytochemical Technique Offering Increased Sensitivity and Lowered Cost with a Streptavidin-Horseradish Peroxidase Conjugate
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使用链霉亲和素-辣根过氧化物酶缀合物提高灵敏度并降低成本的免疫细胞化学技术
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
Pedersen Ca
中科院分区：
文献类型：
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作者：
R. Cartun;Pedersen Ca
摘要免疫细胞化学家寻求的方法，产生高灵敏度和优良的信噪比，同时保持成本效益。我们报告了一个三步标记的抗生物素蛋白生物素（LAB）免疫过氧化物酶，已成功地在我们的实验室在过去的三年。该技术使用商业上可获得的检测试剂，这些试剂是未稀释购买的，并以高稀释度使用，导致每个载玻片的成本为3 - 5美分。已经实现了优异的灵敏度，这允许将一抗和检测系统试剂（市售一抗）稀释至0.1-0.01 μ g/ml的范围，从而节省昂贵的试剂。该方法易于实施，适应忙碌的技术人员的时间表，并提供具有强特异性标记且几乎没有背景干扰的制剂。(The J Histotechnol 12：273，1989）
AbstractImmunocytochemists seek methodologies that yield high sensitivity and excellent signal to noise ratios while remaining cost effective. We report on a three step labeled avidinbiotin (LAB) immunoperoxidase that has been used successfully in our laboratory for the past three years. The technique uses commercially available detection reagents that are purchased undiluted and used at high dilutions, resulting in a cost per slide of 3 – 5 cents. Excellent sensitivity has been achieved, which allows the dilution of primary antibody and detection system reagents (commercially available primary antibodies) to a range of 0.1–0.01 ug/ml, thereby saving on costly reagents. The method is easy to perform, accommodates busy technologists' schedules, and provides preparations with strong specific labeling and virtually no background interference. (The J Histotechnol 12:273, 1989)