The Impact of Bacterial Strain on the Products of Dissimilatory Iron Reduction.
The Impact of Bacterial Strain on the Products of Dissimilatory Iron Reduction.
复制标题
细菌菌株对脱水还原产物的影响。
DOI：
10.1016/j.gca.2009.10.039
复制
发表时间：
2010-01-15
影响因子：
5
通讯作者：
Nealson, Kenneth H.
中科院分区：
文献类型：
--
作者：
Salas, Everett C.;Berelson, William M.;Hammond, Douglas E.;Kampf, Anthony R.;Nealson, Kenneth H.
以希瓦氏菌属的三株细菌为研究对象，研究了特定细菌对异化铁还原产物的影响。菌株CN 32、MR-4和W3-18-1以HFO（水合氧化铁）为末端电子受体，乳酸为有机碳源和能源进行培养。采用X射线粉末衍射、电子显微镜、库仑法和滴定法对铁还原的矿物产物进行了分析。在相同的营养负荷下，菌株CN 32和W3-18-1的铁还原速率相似，约为MR-4的两倍。矿化终产物（次生矿物）的定性和定量评价表明，在还原速率相似但菌株不同的实验中（CN 32和W3-18-1）形成了不同的产物，而在铁还原速率不同且菌株不同的实验中（CN 32和MR-4）形成了相似的产物。菌株CN 32和MR-4还原铁的主要产物是磁铁矿，而W3-18-1则是磁铁矿和碳酸铁氢氧化物水合物（绿色锈）的混合物，这是富锗铁矿的前体。另一个显著的差异是菌株CN 32和MR-4将所有的起始三价铁材料转化为磁铁矿，而W3-18-1没有将大部分Fe 3+转化为可识别的结晶材料。W3-18-1中的生物膜形成比本研究中使用的其他两种菌株更稳健。矿化的差异可能是EPS（或W3-18-1中的另一种细胞产物）可能干扰磁铁矿结晶或促进绿色锈形成的指标。这些结果表明，矿物终产物的相对丰度和这些产物的相对分布强烈依赖于催化铁还原的细菌物种或菌株。
Three bacterial strains from the genus Shewanella were used to examine the influence of specific bacteria on the products of dissimilatory iron reduction. Strains CN32, MR-4 and W3-18-1 were incubated with HFO (hydrous ferric oxide) as the terminal electron acceptor and lactate as the organic carbon and energy source. Mineral products of iron reduction were analyzed using X-ray powder diffraction, electron microscopy, coulometry and susceptometry. Under identical nutrient loadings, iron reduction rates for strains CN32 and W3-18-1 were similar, and about twice as fast as MR-4. Qualitative and quantitative assessment of mineralized end products (secondary minerals) indicated that different products were formed during experiments with similar reduction rates but different strains (CN32 and W3-18-1), and similar products were formed during experiments with different iron reduction rates and different strains (CN32 and MR-4). The major product of iron reduction by strains CN32 and MR-4 was magnetite, while for W3-18-1 it was a mixture of magnetite and iron carbonate hydroxide hydrate (green rust), a precursor to fougerite. Another notable difference was that strains CN32 and MR-4 converted all of the starting ferric iron material into magnetite, while W3-18-1 did not convert most of the Fe3+ into a recognizable crystalline material. Biofilm formation is more robust in W3-18-1 than in the other two strains used in this study. The differences in mineralization may be an indicator that EPS (or another cellular product from W3-18-1) may interfere with the crystallization of magnetite or facilitate formation of green rust. These results suggest that the relative abundance of mineral end products and the relative distribution of these products are strongly dependent on the bacterial species or strain catalyzing iron reduction.
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影响因子：
5
作者：
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通讯作者：
Banwart, SA
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