HIGH-RESOLUTION H-1 NUCLEAR MAGNETIC-RESONANCE STUDY OF CEREBRAL HYPOXIA INVIVO
HIGH-RESOLUTION H-1 NUCLEAR MAGNETIC-RESONANCE STUDY OF CEREBRAL HYPOXIA INVIVO
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DOI：
10.1073/pnas.80.16.4945
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
PRICHARD, JW
中科院分区：
文献类型：
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作者：
BEHAR, KL;DENHOLLANDER, JA;PRICHARD, JW
通过使用放置在头骨上的表面线圈获得大鼠体内 360.13 MHz 的 1 H-NMR 谱。通过与切除的大鼠脑组织和组织的酸提取物的 1H-NMR 谱进行比较，鉴定了多种代谢物的共振。在体内脑光谱中以 2.3 分钟的时间分辨率监测由于施用低 O2 气体混合物而引起的脑乳酸水平的变化。在核磁共振实验期间同时记录脑电图和心电图。当给予 O2 后，脑乳酸恢复到缺氧前的水平时，记录了缺氧反应的可逆性。实验证明了高分辨率 1H-NMR 在实时监测脑代谢病理生理学方面的适用性。
1H-NMR spectra at 360.13 MHz were obtained of the rat brain in vivo by using a surface coil placed over the skull. Resonances of numerous metabolites were identified by comparison with the 1H-NMR spectra of excised rat brain tissue and acid extracts of the tissue. Changes in cerebral lactate levels resulting from the administration of gas mixtures low in O2 were monoitored in the in vivo brain spectra with a time resolution of 2.3 min. The EEG and ECG were recorded simultaneously during the NMR experiments. Reversibility of the hypoxic response was documented when, upon O2 administration, cerebral lactate returned to its prehypoxic level. The experiments demonstrate the applicability of high-resolution 1H-NMR to monitor pathophysiology of brain metabolism in real time.