Effect of intermittent administration of human parathyroid hormone (1-34) on the mandibular condyle of ovariectomized rats
Effect of intermittent administration of human parathyroid hormone (1-34) on the mandibular condyle of ovariectomized rats
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人甲状旁腺激素（1-34）间歇给药对去卵巢大鼠下颌骨髁突的影响
DOI：
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发表时间：
2000
影响因子：
3.3
通讯作者：
H. Ozawa
中科院分区：
文献类型：
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作者：
M. Nakajima;S. Ejiri;Mikako Tanaka;E. Toyooka;S. Kohno;H. Ozawa
摘要：间歇性施用人甲状旁腺激素（1-34）（PTH）可增加骨质疏松实验动物腰椎和长骨的骨量。然而，PTH 是否对颌骨具有相同的作用仍不清楚。本研究确定了间歇性施用 PTH 对受雌激素缺乏影响的大鼠下颌髁突的影响。 50只6月龄大鼠进行假手术或切除卵巢，然后根据手术程序和激素给药分为五组：假手术+载体（SV）、OVX+载体（OV）、OVX+PTH 6 μg/kg每周一次（OP6-1）、OVX+PTH 60 μg/kg每周一次（OP60-1）和OVX+PTH 20 µg/kg 每周 3 次 (OP20-3)。在预防性给药实验中，每组5只大鼠在手术后立即间歇性注射PTH或媒介物6个月，或者在治疗性给药实验中手术后6个月开始注射。切除下颌骨，并使用共焦激光扫描显微镜和软X射线图像进行骨形态测量。两次实验中，OV组骨量均显着低于SV组（P < 0.01）；此外，根据剂量和频率，OP组的骨量高于OV组，特别是OP20-3组。 OP组矿化表面值显着高于OV组（P < 0.01），而OP组侵蚀表面值不显着高于OV组。这项研究表明，对去势大鼠进行预防性和治疗性间歇性给予 PTH 可增加大鼠下颌髁突的骨形成，而不会加速骨吸收活动。这种合成代谢作用通过每周 3 次 20 μg/kg 的注射模式得到最好的诱导。
Abstract: Intermittent administration of human parathyroid hormone (1-34) (PTH) increases bone mass in lumbar vertebrae and long bones of osteoporotic experimental animals. However, whether PTH has the same effect on jaw bones remains unclear. This study determined the effect of intermittent administration of PTH on rat mandibular condyle affected by estrogen deficiency. Fifty 6-month-old rats were either sham operated or ovariectomized, then divided into five groups depending on surgical procedure and hormone administration: sham + vehicle (SV), OVX + vehicle (OV), OVX + PTH 6 μg/kg once per week (OP6-1), OVX + PTH 60 μg/kg once per week (OP60-1), and OVX + PTH 20 μg/kg three times per week (OP20-3). PTH or vehicle was injected intermittently for 6 months in 5 rats of each group either immediately after surgery in a preventive administration experiment, or injected starting 6 months after surgery in a therapeutic administration experiment. The mandibles were excised, and bone morphometry was performed using confocal laser scanning microscopy and soft X-ray images. In both experiments, the bone volume of the OV groups was significantly lower than that of the SV group (P < 0.01); also, depending on dose and frequency, the bone volume of the OP group was higher than that of the OV group, particularly in the OP20-3 group. The value of mineralized surface of the OP groups was significantly higher than that of the OV group (P < 0.01), whereas the value of eroded surface of the OP groups was not significantly higher than that of the OV group. This study indicates that preventive and therapeutic intermittent administration of PTH in ovariectomized rats increase the bone formation in rat mandibular condyle without accelerating bone resorptive activity. This anabolic effect was best induced by the injection mode of 20 μg/kg three times per week.
影响因子：
4.8
作者：
T. Wronski;C. Yen;H. Qi;L. Dann
通讯作者：
T. Wronski;C. Yen;H. Qi;L. Dann
成骨细胞样细胞响应甲状旁腺激素和脂多糖分泌粒细胞巨噬细胞集落刺激因子。
影响因子：
4.8
作者：
Weir,EC;Insogna,KL;Horowitz,MC
通讯作者：
Horowitz,MC