Colonization of the Human Gut by E. coli and Colorectal Cancer Risk
Colonization of the Human Gut by E. coli and Colorectal Cancer Risk
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DOI：
10.1158/1078-0432.ccr-13-1343
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发表时间：
2014-02-15
影响因子：
11.5
通讯作者：
Darfeuille-Michaud, Arlette
中科院分区：
文献类型：
--
作者：
Bonnet, Mathilde;Buc, Emmanuel;Darfeuille-Michaud, Arlette
目的：肠道菌群可能通过多种机制参与结直肠癌的发生。大肠杆菌是人类肠道微生物群的寄生菌，但一些致病菌株已经获得了诱导慢性炎症和/或产生毒素的能力，例如环调节素，其可以参与致癌过程。在这里，我们分析了E。结肠癌患者粘膜相关大肠杆菌群与临床病理特征的关系。我们评估了结肠癌相关大肠杆菌的致癌特性。大肠杆菌菌株在多发性肠肿瘤（Min）小鼠中的作用。从结肠癌患者的肿瘤和粘膜以及憩室病对照的健康粘膜中定量和表征大肠杆菌。Min小鼠接种结肠癌相关E. coli菌株（11 G5）。结果：感染后7周，结肠息肉的数量增加。与正常组织相比，在肿瘤中观察到大肠杆菌。结肠癌的不良预后因素（肿瘤-淋巴结-转移阶段）与大肠杆菌在粘膜的定植之间的关系。大肠杆菌。致病性环调节蛋白阳性E.大肠杆菌菌株在III/IV期结肠癌患者的粘膜上比I期结肠癌患者的粘膜上更普遍。指数和E.大肠杆菌定植水平与远离肿瘤的粘膜显著相关。感染E.大肠杆菌11 G5株可见结肠息肉数明显增加。大肠杆菌可能是大肠癌发病的辅助因子。（C）2013年AACR。
Purpose: The intestinal microbiota is potentially involved in the development of colorectal carcinoma via various mechanisms. Escherichia coli are commensal bacteria of the human gut microbiota, but some pathogenic strains have acquired the ability to induce chronic inflammation and/or produce toxins, such as cyclomodulin, which could participate in the carcinogenesis process. Here, we analyzed the E. coli population associated with mucosa of patients with colon cancer in relation to clinicopathologic characteristics. We assessed carcinogenic properties of a colon cancer-associated E. coli strain in multiple intestinal neoplasia (Min) mice.Experimental design: Mucosa-associated or internalized E. coli were quantified and characterized from tumors and mucosa of patients with colon cancer and the healthy mucosa of diverticulosis controls. Min mice were inoculated with a colon cancer-associated E. coli strain (11G5). The number of colonic polyps was evaluated at 7 weeks after infection.Results: An increased level of mucosa-associated and internalized E. coli was observed in the tumors compared with normal tissue. A relationship between poor prognostic factors for colon cancer (tumor-node-metastasis stage) and colonization of mucosa by E. coli was observed. Pathogenic cyclomodulin-positive E. coli strains were more prevalent on mucosa of patients with stages III/IV than those with stage I colon cancer. Proliferative index and E. coli colonization level of the mucosa distant from the tumor significantly correlated. Min mice infected with the E. coli strain 11G5 displayed a marked increase in the number of visible colonic polyps compared with controls.Conclusion: These findings support that pathogenic E. coli could be a cofactor in pathogenesis of colorectal cancer. (C)2013 AACR.